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BMRB: 7286

2JYZ

Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for CG7054

Authors

Rautureau, G., Jouvensal, L., Vovelle, F.

Assembly

CG7054

Entity

1. CG7054 (polymer, Thiol state: not present), 179 monomers, 19821.93 Da Detail

MDDIVPDVLD AVPAGTIKVI YGDDLEVKQG NELTPTQVKD QPIVSWSGLE GKSNLLTLLM VDPDAPTRQD PKYREILHWS VVNIPGSNEN PSGGHSLADY VGSGPPKDTG LHRYIFLLYR QENKIEETPT ISNTTRTGRL NFNARDFAAK HGLGEPIAAN YYQAQYDDYV PIRNKTIVG

Formula weight

19821.93 Da

Source organism

Drosophila melanogaster

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 92.0 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.0 % (1887 of 2052)	90.7 % (967 of 1066)	92.0 % (737 of 801)	98.9 % (183 of 185)
Backbone	98.3 % (1028 of 1046)	97.8 % (351 of 359)	98.3 % (513 of 522)	99.4 % (164 of 165)
Sidechain	87.3 % (1021 of 1170)	87.1 % (616 of 707)	87.1 % (386 of 443)	95.0 % (19 of 20)
Aromatic	50.0 % (71 of 142)	56.3 % (40 of 71)	42.0 % (29 of 69)	100.0 % (2 of 2)
Methyl	99.0 % (202 of 204)	99.0 % (101 of 102)	99.0 % (101 of 102)

1. CG7054

MDDIVPDVLD AVPAGTIKVI YGDDLEVKQG NELTPTQVKD QPIVSWSGLE GKSNLLTLLM VDPDAPTRQD PKYREILHWS VVNIPGSNEN PSGGHSLADY VGSGPPKDTG LHRYIFLLYR QENKIEETPT ISNTTRTGRL NFNARDFAAK HGLGEPIAAN YYQAQYDDYV PIRNKTIVG

Show sample condition

Sample #1

Temperature 293 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	CG7054	[U-13C; U-15N]	protein	1.5 mM
2	K phosphate pH 6.5		buffer	50 mM
3	DSS			1 mM
4	D2O			10 %
5	NaN3			0.02 %

Sample #2

Temperature 293 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	CG7054	[U-15N]	protein	1.5 mM
7	K phosphate pH 6.5		buffer	50 mM
8	DSS			1 mM
9	D2O			10 %
10	NaN3			0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 1 Detail

Release date

2007-04-25

Citation

1H, 15N and 13C resonance assignments of CG7054, a new PEBP from Drosophila melanogaster
Rautureau, G., Jouvensal, L., Schoentgen, F., Vovelle, F.
J. Biomol. NMR (2007), 38, 187-187, PubMed 17396237 , DOI 10.1007/s10858-006-9112-z , Abstract

Related entities 1. CG7054, : 1 : 1 : 219 entities Detail

Experiments performed 13 experiments Detail

1 1H15N HSQC