BMRBj - BMREntryMaster

Found 1 Document (1.222 seconds)

BMRB: 7289

Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein

Authors

Bruns, K., Studtrucker, N., Sharma, A., Fossen, T., Mitzner, D., Eissmann, A., Tessmer, U., Henklein, P., Wray, V., Schubert, U.

Assembly

PB(1-40)

Entity

1. PB(1-40) (polymer, Thiol state: not present), 40 monomers, 4592.050 Da Detail

MGQEQDTPWI LSTGHISTQK RQDGQQTPKL EHRNSTRLMG

Formula weight

4592.05 Da

Source organism

Influenza A virus (A/Puerto Rico/8/1934(H1N1))

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.6 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	99.6 % (249 of 250)	99.6 % (249 of 250)
Backbone	98.8 % (81 of 82)	98.8 % (81 of 82)
Sidechain	100.0 % (168 of 168)	100.0 % (168 of 168)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (15 of 15)	100.0 % (15 of 15)

1. PB(1-40)

MGQEQDTPWI LSTGHISTQK RQDGQQTPKL EHRNSTRLMG

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 300 (±0) K, pH 3 (±0)

#	Name	Type	Concentration
1	PB(1-40)	protein	2 mM
2	Trifluoroethanol	solvent	50 % v/v
3	water	solvent	50 % v/v

Release date

2006-10-30

Citation

Structural characterization and oligomerization of PB1-F2, a proapoptotic influenza A virus protein
Bruns, K., Studtrucker, N., Sharma, A., Fossen, T., Mitzner, D., Eissmann, A., Tessmer, U., Roder, R., Henklein, P., Wray, V., Schubert, U.
J. Biol. Chem. (2007), 282, 353-363, PubMed 17052982 , DOI 10.1074/jbc.M606494200 , Abstract

Related entities 1. PB(1-40), : 1 : 1 : 53 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7289_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7289_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	289		100.0 (chain: 1, length: 40)
1	Chemical shift references	chemical_shift_referencing	OK	1		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 40, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 289
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
20	LYS	HZ1	7.603
20	LYS	HZ2	7.603
20	LYS	HZ3	7.603
29	LYS	HZ1	7.603
29	LYS	HZ2	7.603
29	LYS	HZ3	7.603

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	250	249	99.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	82	81	98.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	168	168	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	17	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	10	100.0

Keywords Influenza A virus, PB1-F2, Structure and oligomerization

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.222 seconds)

BMRB: 7289

Sample

Related entities 1. PB(1-40), : 1 : 1 : 53 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail