BMRBj - BMREntryMaster

Found 1 Document (1.031 seconds)

BMRB: 7290

Structural characterization and oligomerization of PB1-F2, a pro-apoptotic influenza A virus protein

Authors

Bruns, K., Studtrucker, N., Sharma, A., Fossen, T., Mitzner, D., Eissmann, A., Tessmer, U., Henklein, P., Wray, V., Schubert, U.

Assembly

PB(30-70)

Entity

1. PB(30-70) (polymer, Thiol state: unknown), 41 monomers, 4977.896 Da Detail

LEHRNSTRLM GHCQKTMNQV VMPKQIVYWK QWLSLRNPIL V

Formula weight

4977.896 Da

Source organism

Influenza A virus (A/Puerto Rico/8/1934(H1N1))

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	96.4 % (270 of 280)	96.4 % (270 of 280)
Backbone	96.3 % (78 of 81)	96.3 % (78 of 81)
Sidechain	96.5 % (192 of 199)	96.5 % (192 of 199)
Aromatic	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	95.8 % (23 of 24)	95.8 % (23 of 24)

1. PB(30-70)

LEHRNSTRLM GHCQKTMNQV VMPKQIVYWK QWLSLRNPIL V

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 300 (±0) K, pH 3 (±0)

#	Name	Type	Concentration
1	PB(30-70)	protein	2 mM
2	Trifluoroethanol	solvent	50 % v/v
3	water	solvent	50 % v/v

Release date

2006-10-30

Citation

Structural characterization and oligomerization of PB1-F2, a proapoptotic influenza A virus protein
Bruns, K., Studtrucker, N., Sharma, A., Fossen, T., Mitzner, D., Eissmann, A., Tessmer, U., Roder, R., Henklein, P., Wray, V., Schubert, U.
J. Biol. Chem. (2007), 282, 353-363, PubMed 17052982 , DOI 10.1074/jbc.M606494200 , Abstract

Related entities 1. PB(30-70), : 2 : 4 : 44 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7290_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7290_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	351		100.0 (chain: 1, length: 41)
1	Chemical shift references	chemical_shift_referencing	OK	1		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 41, Sequence coverage: 100.0%

--------10--------20--------30--------40-
LEHRNSTRLMGHCQKTMNQVVMPKQIVYWKQWLSLRNPILV
|||||||||||||||||||||||||||||||||||||||||
LEHRNSTRLMGHCQKTMNQVVMPKQIVYWKQWLSLRNPILV

Total number of assignments
- ¹H chemical shifts: 351

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
4	ARG	HH11	6.64
4	ARG	HH12	6.64
4	ARG	HH21	6.64
4	ARG	HH22	6.64
8	ARG	HH11	6.695
8	ARG	HH12	6.695
8	ARG	HH21	6.695
8	ARG	HH22	6.695
15	LYS	HZ1	7.618
15	LYS	HZ2	7.618
15	LYS	HZ3	7.618
24	LYS	HZ1	7.622
24	LYS	HZ2	7.622
24	LYS	HZ3	7.622
30	LYS	HZ1	7.606
30	LYS	HZ2	7.606
30	LYS	HZ3	7.606
36	ARG	HH11	6.83
36	ARG	HH12	6.83
36	ARG	HH21	6.83
36	ARG	HH22	6.83

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	280	276	98.6

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	81	80	98.8

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	199	196	98.5

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	27	100.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	20	100.0

Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: 1

Keywords Influenza A virus, PB1-F2, Structure and oligomerization

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Found 1 Document (1.031 seconds)

BMRB: 7290

Sample

Related entities 1. PB(30-70), : 2 : 4 : 44 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail