Backbone assignment and RDCs of L11 in complex with RNA and thiostrepton
MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 24.7 % (649 of 2631) | 11.1 % (155 of 1396) | 34.4 % (366 of 1064) | 74.9 % (128 of 171) |
Backbone | 40.1 % (606 of 1510) | 19.7 % (128 of 650) | 48.3 % (350 of 725) | 94.8 % (128 of 135) |
Sidechain | 11.2 % (141 of 1256) | 3.6 % (27 of 746) | 24.1 % (114 of 474) | 0.0 % (0 of 36) |
Aromatic | 2.5 % (8 of 326) | 3.1 % (5 of 163) | 2.2 % (3 of 135) | 0.0 % (0 of 28) |
Methyl | 13.4 % (25 of 186) | 4.3 % (4 of 93) | 22.6 % (21 of 93) |
1. ribosomal L11 protein
MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D2. 23S GAR RNA
GGGCAGGAUG UAGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGCCC3. Thiostrepton antibiotic
XTXXXXXIAX AXXTemperature 298 (±0.1) K, pH 6.1 (±0.05), Details ternary complex
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ribosomal L11 protein | [U->95% 2D; U->98% 15N; U->98% 13C] | protein | 0.3 mM |
2 | 23S GAR RNA | rna | 0.3 mM | |
3 | Thiostrepton antibiotic | antibiotic | 0.3 mM | |
4 | potassium phosphate | buffer | 20 mM | |
5 | potassium chloride | salt | 200 mM | |
6 | complete | protease inhibitoir | ||
7 | superase | rnase inhibitor |