Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDA AYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 34.4 % (374 of 1088) | 18.1 % (102 of 565) | 48.4 % (206 of 426) | 68.0 % (66 of 97) |
Backbone | 61.2 % (340 of 556) | 38.6 % (73 of 189) | 72.6 % (201 of 277) | 73.3 % (66 of 90) |
Sidechain | 15.0 % (93 of 621) | 7.7 % (29 of 376) | 26.9 % (64 of 238) | 0.0 % (0 of 7) |
Aromatic | 10.2 % (10 of 98) | 12.2 % (6 of 49) | 8.3 % (4 of 48) | 0.0 % (0 of 1) |
Methyl | 7.8 % (7 of 90) | 4.4 % (2 of 45) | 11.1 % (5 of 45) |
1. L53AI54A
MEHHHHHHEL KNSISDYTEA EFVQLLKEIE KENVAATDDV LDVLLEHFVK ITEHPDGTDA AYYPSDNRDD SPEGIVKEIK EWRAANGKPG FKQGTemperature 298 (±0.1) K, pH 7 (±0.2), Details doubly-labelled (13C/15N) Im7*L53AI54A sample for backbone resonance assignments.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Im7* | [U-13C; U-15N] | protein | 1 (±0.2) mM |
2 | potassium phosphate buffer | 50 mM | ||
3 | sodium sulphate | 400 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |
Temperature 298 (±0.1) K, pH 7 (±0.2), Details singly-labelled (15N) Im7*L53AI54A sample for relaxation data measurement and 15N-edited NOESY experiment.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Im7* | [U-99% 15N] | protein | 1 (±0.2) mM |
7 | potassium phosphate buffer | 50 mM | ||
8 | sodium sulphate | 400 mM | ||
9 | H2O | 90 % | ||
10 | D2O | 10 % |