BMRB: 7327

Homonuclear NMR Assignment of the Pheromone En-2

Authors

Placzek, W.J., Etezady-Esfarjani, T., Herrmann, T., Peti, W., Wuthrich, K.

Assembly

En-2

Entity

1. En-2 (polymer, Thiol state: all disulfide bound), 60 monomers, 6303.759 Da Detail

DIEDFYTSET CPYKNDSQLA WDTCSGGTGN CGTVCCGQCF SFPVSQSCAG MADSNDCPNA

Formula weight

6303.759 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS39:SG
2	disulfide	sing	1:CYS24:SG	1:CYS35:SG
3	disulfide	sing	1:CYS36:SG	1:CYS48:SG
4	disulfide	sing	1:CYS31:SG	1:CYS57:SG

Source organism

Euplotes nobilii

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 65.7 %, Completeness (bb): 58.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	65.7 % (356 of 542)	93.5 % (289 of 309)	28.8 % (67 of 233)
Backbone	58.6 % (174 of 297)	98.4 % (121 of 123)	30.5 % (53 of 174)
Sidechain	62.5 % (187 of 299)	90.3 % (168 of 186)	16.8 % (19 of 113)
Aromatic	49.1 % (28 of 57)	96.6 % (28 of 29)	0.0 % (0 of 28)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. En-2

DIEDFYTSET CPYKNDSQLA WDTCSGGTGN CGTVCCGQCF SFPVSQSCAG MADSNDCPNA

Show sample condition

Sample

pH 6.0 (±0.1), Details 20 mM Sodium Phosphate buffer pH 6.0, 0.9 mM en-2

#	Name	Isotope labeling	Type	Concentration
1	En-2		protein	0.9 (±0.05) mM
2	Sodium Phosphate		buffer	20 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2NSW, Strand ID: A Detail

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Release date

2007-09-16

Citation

Cold-adapted signal proteins: NMR structures of pheromones from the Antarctic ciliate Euplotes nobilii
Placzek, W.J., Etezady-Esfarjani, T., Herrmann, T., Pedrini, B., Peti, W., Alimenti, C., Luporini, P., Wuthrich, K.
IUBMB Life (2007), 59, 578-585, PubMed 17701553 , DOI 10.1080/15216540701258165 , Abstract

Related entities 1. En-2, : 1 : 1 : 6 entities Detail

Experiments performed 4 experiments Detail