BMRBj - BMREntryMaster

Found 1 Document (2.954 seconds)

BMRB: 7358

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the golli myelin basic protein isoform BG21

Authors

Ahmed, M.A. M., Bamm, V.V., Harauz, G., Ladizhansky, V.

Assembly

rmBG21

Entity

1. rmBG21 (polymer, Thiol state: not present), 199 monomers, 21624.15 Da Detail

GNHSGKRELS AEKASKDGEI HRGEAGKKRS VGKLSQTASE DSDVFGEADA IQNNGTSAED TAVTDSKHTA DPKNNWQGAH PADPGNRPHL IRLFSRDAPG REDNTFKDRP SESDELQTIQ EDPTAASGGL DVMASQKRPS QRSKYLATAS TMDHARHGFL PRHRDTGILD SIGRFFSGDR GAPKRGSGKV SLEHHHHHH

Formula weight

21624.15 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 53.3 %, Completeness (bb): 91.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.3 % (1157 of 2169)	36.7 % (418 of 1138)	67.4 % (557 of 827)	89.2 % (182 of 204)
Backbone	91.9 % (1079 of 1174)	86.5 % (353 of 408)	94.3 % (544 of 577)	96.3 % (182 of 189)
Sidechain	21.3 % (250 of 1174)	8.9 % (65 of 730)	43.1 % (185 of 429)	0.0 % (0 of 15)
Aromatic	0.0 % (0 of 136)	0.0 % (0 of 68)	0.0 % (0 of 67)	0.0 % (0 of 1)
Methyl	24.3 % (35 of 144)	15.3 % (11 of 72)	33.3 % (24 of 72)

1. recombinant murin BG21 isoform of golli myelin basic protein

Show sample condition

Sample

Temperature 280 (±1) K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	recombinant murin BG21 isoform of golli myelin basic protein	[U-100% 13C; U-100% 15N]	protein	1 mM
2	Potassium Chloride		salt	100 mM
3	Sodium Azide		salt	0.005 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 3 Detail

Release date

2007-03-05

Citation

The BG21 isoform of Golli myelin basic protein is intrinsically disordered with a highly flexible amino-terminal domain
Ahmed, M.A. M., Bamm, V.V., Harauz, G., Ladizhansky, V.
Biochemistry (2007), 46, 9700-9712, PubMed 17676872 , DOI 10.1021/bi700632x , Abstract

Related entities 1. rmBG21, : 4 entities Detail

Interaction partners 1. rmBG21, : 36 interactors Detail

More details for 36 interactors identified by 7 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7358_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7358_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	1091		96.5 (chain: 1, length: 199)
1	Chemical shift references	chemical_shift_referencing	OK	3		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 199, Sequence coverage: 96.5%
- Total number of assignments
  - ¹H chemical shifts: 353
  - ¹³C chemical shifts: 546
  - ¹⁵N chemical shifts: 192
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
72	PRO	N	138.3
81	PRO	N	137.9
84	PRO	N	138.0
88	PRO	N	136.9
99	PRO	N	136.2
110	PRO	N	137.7
123	PRO	N	138.0
139	PRO	N	137.6
161	PRO	N	136.3
183	PRO	N	136.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1138	353	31.0
¹³C chemical shifts	827	546	66.0
¹⁵N chemical shifts	220	182	82.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	408	353	86.5
¹³C chemical shifts	398	374	94.0
¹⁵N chemical shifts	189	182	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	730	0	0.0
¹³C chemical shifts	429	172	40.1
¹⁵N chemical shifts	31	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	0	0.0
¹³C chemical shifts	74	19	25.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	0	0.0
¹³C chemical shifts	67	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords chemical shift indexing, genes of oligodendrocyte lineage (Golli), heteronuclear single quantum coherence (HSQC), intrinsically disordered protein, myelin basic protein (MBP), relaxation, solution NMR, Golli, MBP, Resonance Assignment

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Found 1 Document (2.954 seconds)

BMRB: 7358

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. rmBG21, : 4 entities Detail

Interaction partners 1. rmBG21, : 36 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail