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NMR Structure of RRM-1 of Yeast NPL3 Protein
Authors
Deka, P., Bucheli, M., Skrisovska, L., Allain, F.H., Moore, C., Buratowski, S., Varani, G.
Assembly
Nucleolar protein 3
Entity
1. Nucleolar protein 3 (polymer, Thiol state: not present), 74 monomers, 8346.338 Da Detail

ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSK


Formula weight
8346.338 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Refine. method
TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 82.0 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All82.0 % (730 of 890)84.5 % (392 of 464)74.9 % (263 of 351)100.0 % (75 of 75)
Backbone96.1 % (417 of 434)97.3 % (143 of 147)94.0 % (205 of 218)100.0 % (69 of 69)
Sidechain71.1 % (374 of 526)78.5 % (249 of 317)58.6 % (119 of 203)100.0 % (6 of 6)
Aromatic18.5 % (17 of 92)37.0 % (17 of 46) 0.0 % (0 of 46)
Methyl89.7 % (70 of 78)100.0 % (39 of 39)79.5 % (31 of 39)

1. Nucleolar protein 3

ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSK

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Nucleolar_protein_3[U-99% 13C; U-99%15N]1.0 (±0.05) mM
2Potassium phosphate20 mM
3potassium chloride20 mM

LACS Plot; CA
Referencing offset: -0.25 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.25 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.05 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 2.39 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2OSQ, Strand ID: A Detail


Release date
2008-05-05
Citation
Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals
Deka, P., Bucheli, M., Moore, C., Buratowski, S., Varani, G.
J. Mol. Biol. (2008), 375, 136-150, PubMed 18022637 , DOI 10.1016/j.jmb.2007.09.029 ,
Related entities 1. Nucleolar protein 3, : 1 : 6 : 392 entities Detail
Interaction partners 1. Nucleolar protein 3, : 63 interactors Detail
Experiments performed 3 experiments Detail
nullKeywords MRNA, NPL3, RNA-BINDING, RRM, SR PROTEIN