Backbone 1H, 13C, and 15N Chemical Shift Assignments for MxiD in complex with MxiM
SSSNSEKEWH IVPVSKDYFS IPNDLLWSFN TTNKSINVYS KCISGKAVYS FNAGKFMGNF NVKEVDGCFM DAQKIAIDKL FSMLKDGVVL KGNKINDTIL IEKDGEVKLK LIRGI
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 8.8 % (174 of 1976) | 5.2 % (53 of 1028) | 10.5 % (81 of 769) | 22.3 % (40 of 179) |
Backbone | 16.2 % (159 of 984) | 12.8 % (43 of 336) | 15.6 % (76 of 486) | 24.7 % (40 of 162) |
Sidechain | 4.4 % (50 of 1148) | 1.3 % (9 of 692) | 9.3 % (41 of 439) | 0.0 % (0 of 17) |
Aromatic | 0.0 % (0 of 166) | 0.0 % (0 of 83) | 0.0 % (0 of 81) | 0.0 % (0 of 2) |
Methyl | 2.8 % (5 of 178) | 3.4 % (3 of 89) | 2.2 % (2 of 89) |
1. MxiD(525-570)
GSHMKESSYY NTAEYKSLIS EREIQKTTQI IPSETTLLED EKSLVSYLNY2. MxiM(28-142)
SSSNSEKEWH IVPVSKDYFS IPNDLLWSFN TTNKSINVYS KCISGKAVYS FNAGKFMGNF NVKEVDGCFM DAQKIAIDKL FSMLKDGVVL KGNKINDTIL IEKDGEVKLK LIRGISolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 301 (±0.5) K, pH 7.5 (±0.1), Details 20 mM HEPES and 0.5 mM of dodecylmaltosid were present in solution.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MxiD(525-570) | [U-95% 13C; U-95% 15N] | 0.2 - 0.3 mM | |
2 | MxiM(28-142) | 0.2 - 0.3 mM | ||
3 | HEPES | natural abundance | 20 mM | |
4 | dodecylmaltosid | natural abundance | 0.5 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | 5 % |