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BMRB: 7432

2KQK

Structural characterization of IscU and its interaction with HscB

Authors

Kim, J., Fuzery, A.K., Marco, T., William, W.M., Markley, J.L.

Assembly

IscU(D39A)

Entity

1. IscU(D39A) (polymer, Thiol state: all free), 128 monomers, 13804.42 Da Detail

MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGAV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA EDAIKAAIAD YKSKREAK

Formula weight

13804.42 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 88.3 %, Completeness: 33.0 %, Completeness (bb): 58.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.0 % (469 of 1423)	19.1 % (141 of 737)	39.9 % (220 of 552)	80.6 % (108 of 134)
Backbone	58.4 % (444 of 760)	46.4 % (121 of 261)	57.3 % (215 of 375)	87.1 % (108 of 124)
Sidechain	15.9 % (124 of 782)	4.2 % (20 of 476)	35.1 % (104 of 296)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 72)	0.0 % (0 of 36)	0.0 % (0 of 35)	0.0 % (0 of 1)
Methyl	14.8 % (21 of 142)	7.0 % (5 of 71)	22.5 % (16 of 71)

1. IscU(D39A)

MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGAV MKLQIKVNDE GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA EDAIKAAIAD YKSKREAK

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details The sample used for resonance assignment of IscU(D39A)

#	Name	Isotope labeling	Concentration
1	IscU(D39A)	[U-13C; U-15N]	2.5 mM
2	TRIS	natural abundance	20 mM
3	DTT	natural abundance	5-10 mM
4	EDTA	natural abundance	0.5 mM
5	sodium chloride	natural abundance	150 mM
6	DSS	natural abundance	0.7 mM
7	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 8, Details The sample used for relaxation rates and heteronuclear NOE measurements of IscU(D39A)

#	Name	Isotope labeling	Concentration
8	IscU(D39A)	[U-15N]	0.6-0.7 mM
9	TRIS	natural abundance	20 mM
10	DTT	natural abundance	5-10 mM
11	EDTA	natural abundance	0.5 mM
12	sodium chloride	natural abundance	150 mM
13	DSS	natural abundance	0.7 mM
14	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Heteronucl. T₁

105 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 8 Detail

Heteronucl. T₂

105 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 8 Detail

Heteronucl. NOE

105 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 8 Detail

Heteronucl. T₁/T₂

105 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 8 Detail

Release date

2009-08-19

Citation

Structure and dynamics of the iron-sulfur cluster assembly scaffold protein IscU and its interaction with the cochaperone HscB
Kim, J., Fuzery, A.K., Tonelli, M., Ta, D.T., Westler, W.M., Vickery, L.E., Markley, J.L.
Biochemistry (2009), 48, 6062-6071, PubMed 19492851 , DOI 10.1021/bi9002277 , Abstract

Related entities 1. IscU(D39A), : 1 : 4 : 94 entities Detail

Interaction partners 1. IscU(D39A), : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC