BMRB: 10008

Solution structure of C-terminal fibronectin type III domain of mouse 1700129L13Rik protein

Authors

Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

C-terminal FN3 domain of 1700129L13Rik protein

Entity

1. C-terminal FN3 domain of 1700129L13Rik protein (polymer, Thiol state: all free), 123 monomers, 13016.46 Da Detail

GSSGSSGPGA PSTVRISKNV DGIHLSWEPP TSPSGNILEY SAYLAIRTAQ MQDNPSQLVF MRIYCGLKTS CTVTAGQLAN AHIDYTSRPA IVFRISAKNE KGYGPATQIR WLQGNSKSGP SSG

Formula weight

13016.46 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 88.9 %, Completeness (bb): 87.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.9 % (1204 of 1355)	89.5 % (629 of 703)	88.7 % (465 of 524)	85.9 % (110 of 128)
Backbone	87.1 % (627 of 720)	88.0 % (220 of 250)	87.1 % (310 of 356)	85.1 % (97 of 114)
Sidechain	91.0 % (678 of 745)	90.3 % (409 of 453)	92.1 % (256 of 278)	92.9 % (13 of 14)
Aromatic	87.0 % (80 of 92)	87.0 % (40 of 46)	86.4 % (38 of 44)	100.0 % (2 of 2)
Methyl	96.7 % (116 of 120)	96.7 % (58 of 60)	96.7 % (58 of 60)

1. FN3 domain

GSSGSSGPGA PSTVRISKNV DGIHLSWEPP TSPSGNILEY SAYLAIRTAQ MQDNPSQLVF MRIYCGLKTS CTVTAGQLAN AHIDYTSRPA IVFRISAKNE KGYGPATQIR WLQGNSKSGP SSG

Show sample condition

Sample

Pressure 1 (±0.03) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	FN3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	200 mM
4	d-DTT		salt	2 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.38 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WFT, Strand ID: A Detail

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Release date

2006-11-05

Citation

Leukocyte antigen-related protein tyrosine phosphatase receptor: a small ectodomain isoform functions as a homophilic ligand and promotes neurite outgrowth
Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.
J. Neurosci. (2003), 23, 3353-3363, PubMed 12716943 , DOI: , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. C-terminal FN3 domain of 1700129L13Rik protein, : 1 : 1 : 97 entities Detail

Interaction partners 1. C-terminal FN3 domain of 1700129L13Rik protein, : 13 interactors Detail

Experiments performed 2 experiments Detail