BMRB: 11048

RecT-bound ssDNA

Authors

Shibata, T., Ito, Y.

Assembly

5'-D(*DTP*DAP*DCP*DG)-3' 10 structures

Entity

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3') (polymer, Thiol state: not present), 4 monomers, 1253.802 × 10 Da Detail

TACG

2. ecRecT (polymer, Thiol state: unknown), 269 monomers, 29720.69 Da Detail

MTKQPPIAKA DLQKTQGNRA PAAVKNSDVI SFINQPSMKE QLAAALPRHM TAERMIRIAT TEIRKVPALG NCDTMSFVSA IVQCSQLGLE PGSALGHAYL LPFGNKNEKS GKKNVQLIIG YRGMIDLARR SGQIASLSAR VVREGDEFSF EFGLDEKLIH RPGENEDAPV THVYAVARLK DGGTQFEVMT RKQIELVRSL SKAGNNGPWV THWEEMAKKT AIRRLFKYLP VSIEIQRAVS MDEKEPLTID PADSSVLTGE YSVIDNSEE

Total weight

42258.71 Da

Max. entity weight

29720.69 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 4.8 %, Completeness: 4.3 %, Completeness (bb): 6.7 % Detail

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Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	1H
All	4.3 % (72 of 1663)	4.3 % (72 of 1663)
Backbone	6.7 % (38 of 570)	6.7 % (38 of 570)
Sidechain	3.1 % (34 of 1093)	3.1 % (34 of 1093)
Aromatic	6.7 % (6 of 90)	6.7 % (6 of 90)
Methyl	1.3 % (2 of 153)	1.3 % (2 of 153)

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3')

TACG

2. ecRecT

MTKQPPIAKA DLQKTQGNRA PAAVKNSDVI SFINQPSMKE QLAAALPRHM TAERMIRIAT TEIRKVPALG NCDTMSFVSA IVQCSQLGLE PGSALGHAYL LPFGNKNEKS GKKNVQLIIG YRGMIDLARR SGQIASLSAR VVREGDEFSF EFGLDEKLIH RPGENEDAPV THVYAVARLK DGGTQFEVMT RKQIELVRSL SKAGNNGPWV THWEEMAKKT AIRRLFKYLP VSIEIQRAVS MDEKEPLTID PADSSVLTGE YSVIDNSEE

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*DTP*DAP*DCP*DG)-3')	natural abundance	DNA	1 mM
2	ecRecT	natural abundance	protein	0.1 mM
3	NaCl	natural abundance	salt	100 mM
4	D2O		solvent	100 %

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Release date

2009-10-14

Citation

A non-canonical DNA structure enables homologous recombination in various genetic systems
Masuda, T., Ito, Y., Terada, T., Shibata, T., Mikawa, T.
J. Biol. Chem. (2009), 284, 30230-30239, PubMed 19729448 , DOI 10.1074/jbc.M109.043810 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 2. ecRecT, : 2 : 1 entities Detail

Interaction partners 2. ecRecT, : 7 interactors Detail

Experiments performed 5 experiments Detail