BMRBj - BMREntryMaster

Found 1 Document (1.107 seconds)

BMRB: 11073

2RQF

Solution structure of juvenile hormone binding protein from silkworm in complex with JH III

Authors

Suzuki, R., Fujimoto, Z., Shiotsuki, T., Momma, M., Tase, A., Yamazaki, T.

Assembly

JHBP-JH III complex

Entity

1. hJHBP (polymer, Thiol state: free and disulfide bound), 227 monomers, 24756.85 Da Detail

GSDGDALLKP CKLGDMQCLS SATEQFLEKT SKGIPQYDIW PIDPLVVTSL DVIAPSDAGI VIRFKNLNIT GLKNQQISDF QMDTKAKTVL LKTKADLHIV GDIVIELTEQ SKSFTGLYTA DTNVIGAVRY GYNLKNDDNG VQHFEVQPET FTCESIGEPK ITLSSDLSSA LEKDSGNNSL EPDMEPLKTL RQAAICKIAE ACYISVVHNI RASAKILPAS SFFENLN

2. JH3 (non-polymer), 266.376 Da

Total weight

25023.227 Da

Max. entity weight

24756.85 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS18:SG
2	disulfide	sing	1:CYS153:SG	1:CYS196:SG

Source organism

Bombyx mori

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.1 %, Completeness (bb): 98.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.1 % (2546 of 2595)	97.8 % (1311 of 1341)	98.2 % (996 of 1014)	99.6 % (239 of 240)
Backbone	98.8 % (1326 of 1342)	97.8 % (447 of 457)	99.3 % (663 of 668)	99.5 % (216 of 217)
Sidechain	97.6 % (1432 of 1467)	97.7 % (864 of 884)	97.3 % (545 of 560)	100.0 % (23 of 23)
Aromatic	93.1 % (134 of 144)	93.1 % (67 of 72)	93.0 % (66 of 71)	100.0 % (1 of 1)
Methyl	98.6 % (278 of 282)	97.9 % (138 of 141)	99.3 % (140 of 141)

1. hJHBP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	hJHBP	[U-15N]	0.6 ~ 1.0 mM
2	JH III	natural abundance	0.6 ~ 1.0 mM
3	sodium phosphate	natural abundance	45 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
7	JH III	natural abundance	0.6 ~ 1.0 mM
8	sodium phosphate	natural abundance	45 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl carbons	external	direct	1.0
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.29 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RQF, Strand ID: A Detail

Release date

2009-10-13

Citation 1

Structural basis for juvenile hormone perception by JHBP in haemolymph
Suzuki, R., Fujimoto, Z., Shiotsuki, T., Momma, M., Tase, A., Miyazawa, M., Yamazaki, T.

Show more 1 citaion

Citation 2

NMR assignments of juvenile hormone binding protein in complex with JH III
Suzuki, R., Tase, A., Fujimoto, Z., Shiotsuki, T., Yamazaki, T.
Biomol. NMR Assign. (2009), 3, 73-76, PubMed 19636950 , DOI 10.1007/s12104-009-9144-x , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 1 entries Detail

BMRB: 16021 released on 2009-01-27
Title NMR assignments of juvenile hormone binding protein in complex with JH III

Related entities 1. hJHBP, : 1 : 3 : 3 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	hJHBP	[U-15N]	0.6 ~ 1.0 mM
2	JH III	natural abundance	0.6 ~ 1.0 mM
3	sodium phosphate	natural abundance	45 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

2 2D 1H-15N HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

3 2D 1H-13C HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

4 3D HNCO

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
7	JH III	natural abundance	0.6 ~ 1.0 mM
8	sodium phosphate	natural abundance	45 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

5 3D CBCA(CO)NH

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
7	JH III	natural abundance	0.6 ~ 1.0 mM
8	sodium phosphate	natural abundance	45 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

6 3D HNCA

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
7	JH III	natural abundance	0.6 ~ 1.0 mM
8	sodium phosphate	natural abundance	45 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

7 3D HCABGCO

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

8 3D HCCH-TOCSY

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

9 3D 15N-separated HOHAHA-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	hJHBP	[U-15N]	0.6 ~ 1.0 mM
2	JH III	natural abundance	0.6 ~ 1.0 mM
3	sodium phosphate	natural abundance	45 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

10 3D 15N-separated NOESY-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	hJHBP	[U-15N]	0.6 ~ 1.0 mM
2	JH III	natural abundance	0.6 ~ 1.0 mM
3	sodium phosphate	natural abundance	45 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

11 3D 13C/15N-separated NOESY-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

12 3D 13C-separated NOESY-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

13 3D 13C/13C-separated NOESY-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

14 2D 13C-filtered COSY

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

15 2D 13C-filtered NOESY

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

16 3D 13C-filtered/13C-selected NOESY-HSQC

Instrument

Bruker DMX - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	hJHBP	[U-13C; U-15N]	0.6 ~ 1.0 mM
12	JH III	natural abundance	0.6 ~ 1.0 mM
13	sodium phosphate	natural abundance	45 mM
14	D2O	natural abundance	100 %

nullKeywords juvenile hormone

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Found 1 Document (1.107 seconds)

BMRB: 11073

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. hJHBP, : 1 : 3 : 3 entities Detail

Experiments performed 16 experiments Detail

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