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BMRB: 11159


11159
2YUR
Solution structure of the Ring finger of human Retinoblastoma-binding protein 6
Authors
Abe, H., Miyamoto, K., Tochio, N., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.
Assembly
Retinoblastoma-binding protein 6
Entity
1. UNP residues 249-309, C3HC4 domain (polymer, Thiol state: all other bound), 74 monomers, 7689.405 Da Detail

GSSGSSGEDD PIPDELLCLI CKDIMTDAVV IPCCGNSYCD ECIRTALLES DEHTCPTCHQ NDVSPDALSG PSSG


2. ZN (non-polymer), 65.409 × 2 Da
Total weight
7820.2227 Da
Max. entity weight
7689.405 Da
Entity Connection
na 8 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS18:SG2:ZN1:ZN
2nasing1:CYS21:SG2:ZN1:ZN
3nasing1:CYS33:SG2:ZN1:ZN
4nasing1:CYS34:SG2:ZN1:ZN
5nasing1:CYS39:SG2:ZN1:ZN
6nasing1:CYS42:SG2:ZN1:ZN
7nasing1:CYS55:SG2:ZN1:ZN
8nasing1:CYS58:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.6 %, Completeness: 94.8 %, Completeness (bb): 92.8 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All94.8 % (705 of 744)94.0 % (358 of 381)96.6 % (282 of 292)91.5 % (65 of 71)
Backbone92.8 % (401 of 432)89.9 % (133 of 148)95.4 % (206 of 216)91.2 % (62 of 68)
Sidechain97.4 % (370 of 380)96.6 % (225 of 233)98.6 % (142 of 144)100.0 % (3 of 3)
Aromatic100.0 % (16 of 16)100.0 % (8 of 8)100.0 % (8 of 8)
Methyl100.0 % (70 of 70)100.0 % (35 of 35)100.0 % (35 of 35)

1. UNP residues 249-309, C3HC4 domain

GSSGSSGEDD PIPDELLCLI CKDIMTDAVV IPCCGNSYCD ECIRTALLES DEHTCPTCHQ NDVSPDALSG PSSG

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.18mM {Protein;} 20mM {d-Tris-HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 0.05mM Zncl2+1mM {IDA;} 90% H2O, 10% D2O


#NameIsotope labelingTypeConcentration
1UNP residues 249-309, C3HC4 domain[U-13C; U-15N]protein1.18 mM
2d-Tris-HClnatural abundancebuffer20 mM
3NaClnatural abundancesalt100 mM
4d-DTTnatural abundancesalt1 mM
5NaN3natural abundancesalt0.02 %
6ZnCl2natural abundancesalt0.05 mM
7IDAnatural abundancesalt1 mM
8H2Osolvent90 %
9D2Osolvent10 %

LACS Plot; CA
Referencing offset: 0.0 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.0 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.15 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2YUR, Strand ID: A Detail

Release date
2011-05-04
Citation
Solution structure of RING finger-like domain of retinoblastoma-binding protein-6 (RBBP6) suggests it functions as a U-box
Abe, H., Miyamoto, K., Tochio, N., Tomizawa, T., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.
J. Biol. Chem. (2012), 287, 7146-7158, PubMed 22130672 , DOI 10.1074/jbc.M110.217059 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 17466 released on 2011-12-11
    Title Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)
  BMRB: 17772 released on 2011-12-05
    Title Solution structure of the RING finger-like domain of Retinoblastoma Binding Protein-6 (RBBP6)
Related entities 1. UNP residues 249-309, C3HC4 domain, : 1 : 6 : 73 entities Detail
More details for 80 related entities
Interaction partners 1. UNP residues 249-309, C3HC4 domain, : 31 interactors Detail
More details for 31 interactors identified by 13 citations
Experiments performed 2 experiments Detail
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