BMRB: 15037

Solution structure of human beta-microseminoprotein

Authors

Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.

Assembly

human beta-microseminoprotein

Entity

1. human beta-microseminoprotein (polymer, Thiol state: all disulfide bound), 94 monomers, 10782.21 Da Detail

SCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC TCYETEISCC ILVSTPVGYD KDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII

Formula weight

10782.21 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS50:SG
2	disulfide	sing	1:CYS18:SG	1:CYS42:SG
3	disulfide	sing	1:CYS37:SG	1:CYS73:SG
4	disulfide	sing	1:CYS40:SG	1:CYS49:SG
5	disulfide	sing	1:CYS64:SG	1:CYS87:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 70.8 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	70.8 % (408 of 576)	70.8 % (408 of 576)
Backbone	91.4 % (171 of 187)	91.4 % (171 of 187)
Sidechain	60.9 % (237 of 389)	60.9 % (237 of 389)
Aromatic	30.0 % (12 of 40)	30.0 % (12 of 40)
Methyl	87.2 % (34 of 39)	87.2 % (34 of 39)

1. hMSP

SCYFIPNEGV PGDSTRKCMD LKGNKHPINS EWQTDNCETC TCYETEISCC ILVSTPVGYD KDNCQRIFKK EDCKYIVVEK KDPKKTCSVS EWII

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	beta-microseminoprotein	natural abundance		3.4 ~ 4.2 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2IZ3, Strand ID: A Detail

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Release date

2007-01-09

Citation

Solution structures of human and porcine beta-microseminoprotein
Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.
J. Mol. Biol. (2006), 362, 502-515, PubMed 16930619 , DOI 10.1016/j.jmb.2006.07.029 , Abstract

Entries sharing articles BMRB: 3, Swiss-Prot: 2 entries Detail

Related entities 1. human beta-microseminoprotein, : 1 : 3 : 19 entities Detail

Interaction partners 1. human beta-microseminoprotein, : 1 interactors Detail

Experiments performed 2 experiments Detail