BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	84.4 % (1154 of 1367)	87.3 % (623 of 714)	78.7 % (421 of 535)	93.2 % (110 of 118)
Backbone	93.8 % (636 of 678)	94.8 % (217 of 229)	93.2 % (315 of 338)	93.7 % (104 of 111)
Sidechain	77.6 % (620 of 799)	83.7 % (406 of 485)	67.8 % (208 of 307)	85.7 % (6 of 7)
Aromatic	74.4 % (64 of 86)	90.7 % (39 of 43)	57.1 % (24 of 42)	100.0 % (1 of 1)
Methyl	79.6 % (113 of 142)	84.5 % (60 of 71)	74.6 % (53 of 71)

1. CBD

APKASLRLGF SEYSRISNLI VLHLRKVEEE EDESALKRSE LVNWYLKEIE SEIDSEEELI NKKRIIEKVI HRLTHYDHVL IELTQAGLKG STEGSESYEE DPYLVVNPNY LLED

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
9	MES	natural abundance	50 mM
10	EDTA	natural abundance	1 mM
11	Glycerol	natural abundance	5 %
12	NaCl	natural abundance	500 mM
13	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	CBD	natural abundance	0.5 mM
15	MES	natural abundance	50 mM
16	EDTA	natural abundance	1 mM
17	Glycerol	natural abundance	5 %
18	NaCl	natural abundance	500 mM
19	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KLQ, Strand ID: A Detail

Release date

2010-05-19

Citation

Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6
Wei, Z., Liu, C., Wu, X., Xu, N., Zhou, B., Liang, C., Zhu, G.
J. Biol. Chem. (2010), 285, 12469-12473, PubMed 20202939 , DOI 10.1074/jbc.C109.094599 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 16796 released on 2011-05-18
    Title Solution NMR structure of the Cdt1 binding domain(CBD) in complex with the MCM6 binding domain (MBD)
  Swiss-Prot: Q14566 released on 1997-11-01
    Title MCM6_HUMAN Entity DNA replication licensing factor MCM6

Related entities 1. CBD, : 1 : 1 : 2 : 24 entities Detail

Interaction partners 1. CBD, : 70 interactors Detail

More details for 70 interactors identified by 26 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
9	MES	natural abundance	50 mM
10	EDTA	natural abundance	1 mM
11	Glycerol	natural abundance	5 %
12	NaCl	natural abundance	500 mM
13	D2O	natural abundance	100 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
9	MES	natural abundance	50 mM
10	EDTA	natural abundance	1 mM
11	Glycerol	natural abundance	5 %
12	NaCl	natural abundance	500 mM
13	D2O	natural abundance	100 %

9 3D HCCH-COSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
9	MES	natural abundance	50 mM
10	EDTA	natural abundance	1 mM
11	Glycerol	natural abundance	5 %
12	NaCl	natural abundance	500 mM
13	D2O	natural abundance	100 %

10 3D HBHA(CO)NH

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

11 3D 1H-15N NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
2	MES	natural abundance	50 mM
3	EDTA	natural abundance	1 mM
4	Glycerol	natural abundance	5 %
5	NaCl	natural abundance	500 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	CBD	[U-100% 13C; U-100% 15N]	0.5 mM
9	MES	natural abundance	50 mM
10	EDTA	natural abundance	1 mM
11	Glycerol	natural abundance	5 %
12	NaCl	natural abundance	500 mM
13	D2O	natural abundance	100 %

13 2D 1H-1H NOESY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	CBD	natural abundance	0.5 mM
15	MES	natural abundance	50 mM
16	EDTA	natural abundance	1 mM
17	Glycerol	natural abundance	5 %
18	NaCl	natural abundance	500 mM
19	D2O	natural abundance	100 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_16396_2klq.nef
Input source #2: Coordindates	2klq.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--710-----720-------730-------740-------750-------760-------770-------780-------790-------800-------
APKASLRLGFSEYSRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEE
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
APKASLRLGFSEYSRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRLTHYDHVLIELTQAGLKGSTEGSESYEE
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

810-------820-
DPYLVVNPNYLLED
||||||||||||||
DPYLVVNPNYLLED
-------110----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	114	0	0	100.0

Content subtype: combined_16396_2klq.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1150		100.0 (chain: A, length: 114)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	2013 (1)	noe	95.6 (chain: A, length: 114)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	58 (1)	hbond	36.0 (chain: A, length: 114)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	127 (127)	.	69.3 (chain: A, length: 114)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 114, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 627
  - ¹³C chemical shifts: 414
  - ¹⁵N chemical shifts: 109
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
778	HIS	HD1	7.105

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	714	625	87.5
¹³C chemical shifts	535	414	77.4
¹⁵N chemical shifts	124	109	87.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	229	217	94.8
¹³C chemical shifts	228	212	93.0
¹⁵N chemical shifts	111	103	92.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	485	408	84.1
¹³C chemical shifts	307	202	65.8
¹⁵N chemical shifts	13	6	46.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	61	85.9
¹³C chemical shifts	71	53	74.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	39	90.7
¹³C chemical shifts	42	20	47.6
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 114, Sequence coverage: 95.6%
- Total number of restraints: 2013
- Weight of restraints: 1.0 (2013)
- Potential type of restraints: square-well-parabolic (2013)
- Range of distance target values: 1.27, 2.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 114, Sequence coverage: 36.0%
  - Total number of restraints: 58
  - Weight of restraints: 1.0 (58)
  - Potential type of restraints: square-well-parabolic (58)
  - Range of distance target values: 1.9, 2.85 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 114, Sequence coverage: 69.3%
- Total number of restraints: 127
- Total number of restraints per polymer type: 127
- Weight of restraints: 1.0 (127)
- Potential type of restraints: square-well-parabolic (127)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords CBD structure, Cdt1, DNA replication, Mcm6, Pre-RC

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Found 1 Document (2.517 seconds)

BMRB: 16396

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. CBD, : 1 : 1 : 2 : 24 entities Detail

Interaction partners 1. CBD, : 70 interactors Detail

Experiments performed 13 experiments Detail

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NMR combined restraints 5 contents Detail