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Found 1 Document (0.903 seconds)

BMRB: 16889

2K4I

Solution structure of HIV-2 myrMA bound to di-C4-PI(4,5)P2

Authors

Saad, J.S., Ablan, S.D., Ghanam, R.H., Kim, A., Andrews, K., Nagashima, K., Freed, E.O., Summers, M.F.

Assembly

HIV-2 myrMA bound to di-C4-PI(4,5)P2

Entity

1. HIV-2 (polymer, Thiol state: all free), 135 monomers, 15081.38 Da Detail

XGARNSVLRG KKADELERIR LRPGGKKKYR LKHIVWAANK LDRFGLAESL LESKEGCQKI LTVLDPMVPT GSENLKSLFN TVCVIWCIHA EEKVKDTEGA KQIVRRHLVA ETGTAEKMPS TSRPTAPSSE KGGNY

2. PBU (non-polymer), 634.354 Da

Total weight

15715.734 Da

Max. entity weight

15081.38 Da

Source organism

Human immunodeficiency virus 2

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 72.6 %, Completeness (bb): 76.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.6 % (1134 of 1563)	73.5 % (605 of 823)	66.2 % (399 of 603)	94.9 % (130 of 137)
Backbone	76.6 % (607 of 792)	90.5 % (247 of 273)	60.1 % (235 of 391)	97.7 % (125 of 128)
Sidechain	70.7 % (632 of 894)	65.1 % (358 of 550)	80.3 % (269 of 335)	55.6 % (5 of 9)
Aromatic	61.1 % (44 of 72)	61.1 % (22 of 36)	58.8 % (20 of 34)	100.0 % (2 of 2)
Methyl	93.2 % (138 of 148)	93.2 % (69 of 74)	93.2 % (69 of 74)

1. HIV-2

XGARNSVLRG KKADELERIR LRPGGKKKYR LKHIVWAANK LDRFGLAESL LESKEGCQKI LTVLDPMVPT GSENLKSLFN TVCVIWCIHA EEKVKDTEGA KQIVRRHLVA ETGTAEKMPS TSRPTAPSSE KGGNY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
9	HIV-2 myristoylated matrix protein	natural abundance	0.8 mM
10	potassium phosphate	natural abundance	20 mM
11	DTT	natural abundance	10 mM
12	Glutamate	natural abundance	50 mM
13	Aspartate	natural abundance	50 mM
14	sodium chloride	natural abundance	100 mM
15	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2K4I, Strand ID: A Detail

Release date

2012-08-01

Citation

Structure of the myristylated human immunodeficiency virus type 2 matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in membrane targeting
Saad, J.S., Ablan, S.D., Ghanam, R.H., Kim, A., Andrews, K., Nagashima, K., Soheilian, F., Freed, E.O., Summers, M.F.
J. Mol. Biol. (2008), 382, 434-447, PubMed 18657545 , DOI 10.1016/j.jmb.2008.07.027 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 16888 released on 2012-08-01
    Title Solution structure of the HIV-2 myristoylated Matrix protein
  BMRB: 16887 released on 2010-08-11
    Title Backbone 1H, 13C, 15N Chemical Shift Assignments for HIV-2 unmyristoylated matrix protein

Related entities 1. HIV-2, : 1 : 4 : 83 entities Detail

Interaction partners 1. HIV-2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

3 2D 1H-1H NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
9	HIV-2 myristoylated matrix protein	natural abundance	0.8 mM
10	potassium phosphate	natural abundance	20 mM
11	DTT	natural abundance	10 mM
12	Glutamate	natural abundance	50 mM
13	Aspartate	natural abundance	50 mM
14	sodium chloride	natural abundance	100 mM
15	D2O	natural abundance	100 %

4 3D HNCA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

5 3D HN(CO)CA

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
16	HIV-2 myristoylated matrix protein	[U-13C; U-15N]	0.8 mM
17	potassium phosphate	natural abundance	20 mM
18	DTT	natural abundance	10 mM
19	Glutamate	natural abundance	50 mM
20	Aspartate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	H2O	natural abundance	90 %
23	D2O	natural abundance	10 %

6 3D 1H-15N TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	HIV-2 myristoylated matrix protein	[U-15N]	0.8 mM
2	potassium phosphate	natural abundance	20 mM
3	DTT	natural abundance	10 mM
4	Glutamate	natural abundance	50 mM
5	Aspartate	natural abundance	50 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

nullKeywords Protein

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Found 1 Document (0.903 seconds)

BMRB: 16889

Sample #1

Sample #2

Sample #3

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. HIV-2, : 1 : 4 : 83 entities Detail

Interaction partners 1. HIV-2, : 1 interactors Detail

Experiments performed 6 experiments Detail

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