BMRB: 16951

The Structure of RNA Internal Loops with Tandem AG Pairs: 5'GAGC/3'CGAG

Authors

Hammond, N.B., Kennedy, S.D., Turner, D.H.

Assembly

5'-R(*GP*AP*CP*AP*AP*GP*UP*GP*UP*CP*A)-3'

Entity

1. 5'-R(*GP*AP*CP*AP*AP*GP*UP*GP*UP*CP*A)-3' (polymer, Thiol state: not present), 11 monomers, 3608.166 × 2 Da Detail

GACGAGCGUC A

Total weight

7216.332 Da

Max. entity weight

3608.166 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.5 %, Completeness (bb): 81.1 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	31P
All	85.5 % (153 of 179)	97.9 % (93 of 95)	68.5 % (50 of 73)	90.9 % (10 of 11)
Suger, PO4	81.1 % (107 of 132)	97.0 % (64 of 66)	60.0 % (33 of 55)	90.9 % (10 of 11)
Nucleobase	97.9 % (46 of 47)	100.0 % (29 of 29)	94.4 % (17 of 18)
Aromatic	97.6 % (40 of 41)	100.0 % (23 of 23)	94.4 % (17 of 18)

1. RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')

GACGAGCGUC A

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.1

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')	natural abundance		1 mM
2	sodium chloride	natural abundance		80 mM
3	sodium phosphate	natural abundance		10 mM
4	EDTA	natural abundance		0.5 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 6.1

#	Name	Isotope labeling	Type	Concentration
7	RNA (5'-R(*GP*AP*CP*GP*AP*GP*CP*GP*UP*CP*A)-3')	natural abundance		1 mM
8	sodium chloride	natural abundance		80 mM
9	sodium phosphate	natural abundance		10 mM
10	EDTA	natural abundance		0.5 mM
11	D2O	natural abundance		100 %

Hide sample codition

Release date

2010-07-01

Citation

RNA internal loops with tandem AG pairs: the structure of the 5'GAGU/3'UGAG loop can be dramatically different from others, including 5'AAGU/3'UGAA
Hammond, N.B., Tolbert, B.S., Kierzek, R., Turner, D.H., Kennedy, S.D.
Biochemistry (2010), 49, 5817-5827, PubMed 20481618 , DOI 10.1021/bi100332r , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. 5'-R(*GP*AP*CP*AP*AP*GP*UP*GP*UP*CP*A)-3', : 1 entities Detail

Experiments performed 6 experiments Detail