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BMRB: 17143

2L2N

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the first dsRBD of protein HYL1

Authors

Rasia, R.M., Mateos, J.L., Bologna, N.G., Burdisso, P., Imbert, L., Palatnik, J.F., Boisbouvier, J.

Assembly

HYL1 dsRBD 1

Entity

1. HYL1 dsRBD 1 (polymer, Thiol state: all free), 103 monomers, 11371.70 Da Detail

GHMMTSTDVS SGVSNCYVFK SRLQEYAQKY KLPTPVYEIV KEGPSHKSLF QSTVILDGVR YNSLPGFFNR KAAEQSAAEV ALRELAKSSE LSQCVSQPVH ETG

Formula weight

11371.7 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

rosetta full atom relaxation

Data set

assigned_chemical_shifts, RDCs

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 45.5 %, Completeness (bb): 83.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.5 % (538 of 1182)	29.5 % (181 of 614)	59.7 % (275 of 461)	76.6 % (82 of 107)
Backbone	83.1 % (505 of 608)	76.3 % (158 of 207)	87.8 % (266 of 303)	82.7 % (81 of 98)
Sidechain	17.6 % (118 of 671)	5.7 % (23 of 407)	36.9 % (94 of 255)	11.1 % (1 of 9)
Aromatic	0.0 % (0 of 92)	0.0 % (0 of 46)	0.0 % (0 of 46)
Methyl	17.3 % (18 of 104)	11.5 % (6 of 52)	23.1 % (12 of 52)

1. HYL1 dsRBD 1

GHMMTSTDVS SGVSNCYVFK SRLQEYAQKY KLPTPVYEIV KEGPSHKSLF QSTVILDGVR YNSLPGFFNR KAAEQSAAEV ALRELAKSSE LSQCVSQPVH ETG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	HYL1 dsRBD 1	[U-100% 13C; U-100% 15N]	0.5 mM
2	sodium phosphate	natural abundance	100 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	10 mM
5	sodium azide	natural abundance	0.05 %
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	HYL1 dsRBD 1	[U-100% 13C; U-100% 15N]	0.5 mM
9	sodium phosphate	natural abundance	100 mM
10	sodium chloride	natural abundance	50 mM
11	DTT	natural abundance	10 mM
12	sodium azide	natural abundance	0.05 %
13	C12E5	natural abundance	5 %
14	Hexanol	natural abundance	0.2 %
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514495
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2L2N, Strand ID: A Detail

RDC

292 RDC values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 7.0 Detail

Release date

2010-09-23

Citation

Structure and RNA interactions of the plant MicroRNA processing-associated protein HYL1
Rasia, R.M., Mateos, J., Bologna, N.G., Burdisso, P., Imbert, L., Palatnik, J.F., Boisbouvier, J.
Biochemistry (2010), 49, 8237-8239, PubMed 20735118 , DOI 10.1021/bi100672x , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 17141 released on 2010-09-27
    Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for HYL1-dsRBD2
  Swiss-Prot: O04492 released on 2011-02-08
    Title DRB1_ARATH Entity Double-stranded RNA-binding protein 1

Related entities 1. HYL1 dsRBD 1, : 1 : 2 : 37 entities Detail

Interaction partners 1. HYL1 dsRBD 1, : 7 interactors Detail

More details for 7 interactors identified by 3 citations