Conformation Effects of Base Modification on the Anticodon Stem-loop of Bacillus subtilis tRNATYR
Polymer type: polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 35.3 % (82 of 232) | 36.4 % (48 of 132) | 34.0 % (34 of 100) |
Suger, PO4 | 20.0 % (33 of 165) | 25.6 % (23 of 90) | 13.3 % (10 of 75) |
Nucleobase | 73.1 % (49 of 67) | 59.5 % (25 of 42) | 96.0 % (24 of 25) |
Aromatic | 86.0 % (49 of 57) | 78.1 % (25 of 32) | 96.0 % (24 of 25) |
1. coi6y
GGGGACUGUA XAXCCCCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | i6A,PSU-ASL | natural abundance | 2.0 mM | |
2 | potassium chloride | natural abundance | 10 mM | |
3 | EDTA | natural abundance | 0.02 mM | |
4 | potassium phosphate | natural abundance | 10 mM | |
5 | D2O | natural abundance | 100 % |