H/ACA RNP protein Nhp2p
SKKLNKKVLK TVKKASKAKN VKRGVKEVVK ALRKGEKGLV VIAGDISPAD VISHIPVLCE DHSVPYIFIP SKQDLGAAGA TKRPTSVVFI VPGSNKKKDG KNKEEEYKES FNEVVKEVQA L
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 78.9 % (1137 of 1441) | 75.0 % (570 of 760) | 80.7 % (451 of 559) | 95.1 % (116 of 122) |
Backbone | 95.2 % (680 of 714) | 96.7 % (236 of 244) | 93.5 % (332 of 355) | 97.4 % (112 of 115) |
Sidechain | 67.5 % (567 of 840) | 64.7 % (334 of 516) | 72.2 % (229 of 317) | 57.1 % (4 of 7) |
Aromatic | 14.8 % (8 of 54) | 29.6 % (8 of 27) | 0.0 % (0 of 27) | |
Methyl | 85.1 % (126 of 148) | 93.2 % (69 of 74) | 77.0 % (57 of 74) |
1. Nhp2p
SKKLNKKVLK TVKKASKAKN VKRGVKEVVK ALRKGEKGLV VIAGDISPAD VISHIPVLCE DHSVPYIFIP SKQDLGAAGA TKRPTSVVFI VPGSNKKKDG KNKEEEYKES FNEVVKEVQA LSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HEPES | [U-13C; U-15N] | 20 mM | |
2 | potassium chloride | natural abundance | 200 mM | |
3 | DTT | natural abundance | 1 mM | |
4 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | HEPES | [U-15N] | 20 mM | |
6 | potassium chloride | natural abundance | 200 mM | |
7 | DTT | natural abundance | 1 mM | |
8 | D2O | natural abundance | 10 % | |
9 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | HEPES | [U-13C; U-15N] | 20 mM | |
11 | potassium chloride | natural abundance | 200 mM | |
12 | DTT | natural abundance | 1 mM | |
13 | D2O | natural abundance | 100 % |