Solution structure of the EDC3-LSm domain
GAMGMSVADF YGSNVEVLLN NDSKARGVIT NFDSSNSILQ LRLANDSTKS IVTKDIKDLR ILPKNEIMPK NGTKSPSTNS TKLKSAETYS SKNKWSMDCD EEFDFAANLE KFDKKQVFAE FREKD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 69.0 % (1006 of 1459) | 67.0 % (512 of 764) | 71.2 % (398 of 559) | 70.6 % (96 of 136) |
Backbone | 76.6 % (570 of 744) | 74.2 % (187 of 252) | 78.4 % (290 of 370) | 76.2 % (93 of 122) |
Sidechain | 63.7 % (532 of 835) | 63.5 % (325 of 512) | 66.0 % (204 of 309) | 21.4 % (3 of 14) |
Aromatic | 27.6 % (27 of 98) | 55.1 % (27 of 49) | 0.0 % (0 of 48) | 0.0 % (0 of 1) |
Methyl | 93.0 % (106 of 114) | 94.7 % (54 of 57) | 91.2 % (52 of 57) |
1. EDC3-LSm
GAMGMSVADF YGSNVEVLLN NDSKARGVIT NFDSSNSILQ LRLANDSTKS IVTKDIKDLR ILPKNEIMPK NGTKSPSTNS TKLKSAETYS SKNKWSMDCD EEFDFAANLE KFDKKQVFAE FREKDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EDC3-LSm | [U-100% 15N] | 0.7 mM | |
2 | NaPO4 | natural abundance | 50 mM | |
3 | imidazole | natural abundance | 10 mM | |
4 | NaCl | natural abundance | 150 mM |