BMRBj - BMREntryMaster

Found 1 Document (1.244 seconds)

BMRB: 18466

2LTB

Wild-type FAS1-4

Authors

Underhaug, J., Nielsen, N., Runager, K.

Assembly

Wild-type FAS1-4

Entity

1. Wild-type FAS1-4 (polymer, Thiol state: not present), 135 monomers, 14465.54 Da Detail

AGMGTVMDVL KGDNRFSMLV AAIQSAGLTE TLNREGVYTV FAPTNEAFRA LPPRERSRLL GDAKELANIL KYHIGDEILV SGGIGALVRL KSLQGDKLEV SLKNNVVSVN KEPVAEPDIM ATNGVVHVIT NVLQP

Formula weight

14465.54 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 85.5 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.5 % (1299 of 1519)	84.7 % (663 of 783)	85.0 % (506 of 595)	92.2 % (130 of 141)
Backbone	95.2 % (760 of 798)	94.6 % (261 of 276)	95.4 % (375 of 393)	96.1 % (124 of 129)
Sidechain	78.0 % (658 of 844)	79.3 % (402 of 507)	76.9 % (250 of 325)	50.0 % (6 of 12)
Aromatic	50.0 % (27 of 54)	92.6 % (25 of 27)	7.4 % (2 of 27)
Methyl	97.4 % (189 of 194)	99.0 % (96 of 97)	95.9 % (93 of 97)

1. Wild-type FAS1-4

AGMGTVMDVL KGDNRFSMLV AAIQSAGLTE TLNREGVYTV FAPTNEAFRA LPPRERSRLL GDAKELANIL KYHIGDEILV SGGIGALVRL KSLQGDKLEV SLKNNVVSVN KEPVAEPDIM ATNGVVHVIT NVLQP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 7% stretched polyacrylamide

#	Name	Isotope labeling	Concentration
10	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
11	sodium phosphate	natural abundance	50 mM
12	sodium chloride	natural abundance	100 mM
13	DSS	natural abundance	0.5 mM
14	sodium azide	natural abundance	0.5 %
15	arginine	natural abundance	50 mM
16	glutamate	natural abundance	50 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LTB, Strand ID: A Detail

Release date

2013-08-14

Citation

Mutation in transforming growth factor beta induced protein associated with granular corneal dystrophy type 1 reduces the proteolytic susceptibility through local structural stabilization
Underhaug, J., Kolds, H., Runager, K., Nielsen, J., Srensen, C.S., Kristensen, T., Otzen, D.E., Karring, H., Malmendal, A., Schitt, B., Enghild, J.J., Nielsen, N.
Biochim. Biophys. Acta (2013), 1834, 2812-2822, PubMed 24129074 , DOI 10.1016/j.bbapap.2013.10.008 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18467 released on 2013-08-14
Title FAS1-4, R555W

Related entities 1. Wild-type FAS1-4, : 1 : 1 : 8 : 127 entities Detail

Interaction partners 1. Wild-type FAS1-4, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

5 3D CBCANH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

6 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

7 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

8 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

9 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

11 3D 15N-TOCSY-HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

12 3D 13C-NOESY-HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

13 3D 15N-NOESY-HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

14 2D NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

15 2D IPAP-HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

16 2D IPAP-HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 7% stretched polyacrylamide

#	Name	Isotope labeling	Concentration
10	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
11	sodium phosphate	natural abundance	50 mM
12	sodium chloride	natural abundance	100 mM
13	DSS	natural abundance	0.5 mM
14	sodium azide	natural abundance	0.5 %
15	arginine	natural abundance	50 mM
16	glutamate	natural abundance	50 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

17 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 7% stretched polyacrylamide

#	Name	Isotope labeling	Concentration
10	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
11	sodium phosphate	natural abundance	50 mM
12	sodium chloride	natural abundance	100 mM
13	DSS	natural abundance	0.5 mM
14	sodium azide	natural abundance	0.5 %
15	arginine	natural abundance	50 mM
16	glutamate	natural abundance	50 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

18 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Wild-type_FAS1-4	[U-99% 13C; U-99% 15N]	0.2 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	DSS	natural abundance	0.5 mM
5	sodium azide	natural abundance	0.5 %
6	arginine	natural abundance	50 mM
7	glutamate	natural abundance	50 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

nullKeywords Human, Protein

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Found 1 Document (1.244 seconds)

BMRB: 18466

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Wild-type FAS1-4, : 1 : 1 : 8 : 127 entities Detail

Interaction partners 1. Wild-type FAS1-4, : 3 interactors Detail

Experiments performed 18 experiments Detail

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