Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.2 % (649 of 753) | 86.4 % (338 of 391) | 83.3 % (239 of 287) | 96.0 % (72 of 75) |
Backbone | 97.5 % (384 of 394) | 97.9 % (137 of 140) | 97.9 % (185 of 189) | 95.4 % (62 of 65) |
Sidechain | 77.2 % (321 of 416) | 80.1 % (201 of 251) | 71.0 % (110 of 155) | 100.0 % (10 of 10) |
Aromatic | 11.6 % (10 of 86) | 11.6 % (5 of 43) | 9.5 % (4 of 42) | 100.0 % (1 of 1) |
Methyl | 98.1 % (51 of 52) | 100.0 % (26 of 26) | 96.2 % (25 of 26) |
1. LmCsp
MEQGTVKWFN AEKGFGFIER ENGDDVFVHF SAIQGDGFKS LDEGQAVTFD VEEGQRGPQA ANVQKASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | LmCsp | [U-13C; U-15N] | 0.8 mM | |
2 | potassium phosphate | natural abundance | 50 mM | |
3 | EDTA | natural abundance | 0.1 mM | |
4 | potassium chloride | natural abundance | 100 mM | |
5 | DSS | natural abundance | 0.004 v/v | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |