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BMRB: 18882

2M1W

TICAM-2 TIR domain

Authors

Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.

Assembly

TICAM-2 TIR domain

Entity

1. TICAM-2 TIR domain (polymer), 166 monomers, 19365.68 Da Detail

GPLGSEEVFL KFVILHAEDD TDEALRVQNL LQDDFGIKPG IIFAEMPHGR QHLQNLDDAV NGSAWTILLL TENFLRDTWC NFQFYTSLMN SVNRQHKYNS VIPMRPLNNP LPRERTPFAL QTINALEEES RGFPTQVERI FQESVYKTQQ TIWKETRNMV QRQFIA

Formula weight

19365.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.9 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.9 % (1859 of 2023)	94.2 % (997 of 1058)	88.7 % (692 of 780)	91.9 % (170 of 185)
Backbone	94.6 % (925 of 978)	95.8 % (316 of 330)	94.5 % (464 of 491)	92.4 % (145 of 157)
Sidechain	90.3 % (1087 of 1204)	93.5 % (681 of 728)	85.0 % (381 of 448)	89.3 % (25 of 28)
Aromatic	63.4 % (118 of 186)	86.0 % (80 of 93)	38.9 % (35 of 90)	100.0 % (3 of 3)
Methyl	100.0 % (182 of 182)	100.0 % (91 of 91)	100.0 % (91 of 91)

1. TICAM-2 TIR

GPLGSEEVFL KFVILHAEDD TDEALRVQNL LQDDFGIKPG IIFAEMPHGR QHLQNLDDAV NGSAWTILLL TENFLRDTWC NFQFYTSLMN SVNRQHKYNS VIPMRPLNNP LPRERTPFAL QTINALEEES RGFPTQVERI FQESVYKTQQ TIWKETRNMV QRQFIA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4, Details TICAM-2 TIR (75-235)

#	Name	Isotope labeling	Concentration
1	TICAM-2 TIR	[U-99% 13C; U-99% 15N]	0.5 mM
2	HEPES-NaOH	natural abundance	50 mM
3	DTT	natural abundance	10 mM
4	glycerol	[U-100% 2H]	5 %
5	DSS	natural abundance	0.02 mg/mL

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M1W, Strand ID: A Detail

Release date

2014-01-12

Citation

Structures and interface mapping of the TIR domain-containing adaptor molecules involved in interferon signaling
Enokizono, Y., Kumeta, H., Funami, K., Horiuchi, M., Sarmiento, J., Yamashita, K., Standley, D.M., Matsumoto, M., Seya, T., Inagaki, F.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, 19908-19913, PubMed 24255114 , DOI 10.1073/pnas.1222811110 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18883 released on 2012-12-20
Title TICAM-1 TIR domain structure

Related entities 1. TICAM-2 TIR domain, : 1 : 2 : 17 entities Detail

Interaction partners 1. TICAM-2 TIR domain, : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC