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BMRB: 19366

2MAO

NMR structure of region 2 of E. coli sigmaE

Authors

Campagne, S., Vorholt, J.A., Allain, F.H-T.

Assembly

region 2 of E. coli sigmaE

Entity

1. region 2 of E. coli sigmaE (polymer, Thiol state: not present), 106 monomers, 12230.73 Da Detail

MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFRGDSAF YTWLYRIAVN TAKNYLVAQG RRLELVPRGS HHHHHH

Formula weight

12230.73 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 81.5 %, Completeness (bb): 81.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.5 % (1028 of 1262)	86.5 % (565 of 653)	71.3 % (353 of 495)	96.5 % (110 of 114)
Backbone	81.1 % (511 of 630)	91.6 % (196 of 214)	68.7 % (215 of 313)	97.1 % (100 of 103)
Sidechain	83.6 % (613 of 733)	84.1 % (369 of 439)	82.7 % (234 of 283)	90.9 % (10 of 11)
Aromatic	64.1 % (82 of 128)	78.1 % (50 of 64)	49.2 % (31 of 63)	100.0 % (1 of 1)
Methyl	91.4 % (117 of 128)	90.6 % (58 of 64)	92.2 % (59 of 64)

1. entity

MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFRGDSAF YTWLYRIAVN TAKNYLVAQG RRLELVPRGS HHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

LACS Plot; CA

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 18 models in PDB: 2MAO, Strand ID: A Detail

Release date

2014-02-09

Citation

Structural basis for -10 promoter element melting by environmentally induced sigma factors
Campagne, S., Marsh, M.E., Capitani, G., Vorholt, J.A., Allain, F.
Nat. Struct. Mol. Biol. (2014), 21, 269-276, PubMed 24531660 , DOI 10.1038/nsmb.2777 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34172 released on 2017-08-16
    Title NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA
  BMRB: 19367 released on 2014-02-09
    Title Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 non template element TGTCAAA

Related entities 1. region 2 of E. coli sigmaE, : 1 : 1 : 4 : 5 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

3 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

4 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

5 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

9 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	NaPO4	natural abundance		10 mM
5	NaCl	natural abundance		50 mM

nullKeywords ECF sigma factor