BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	52.6 % (583 of 1109)	51.0 % (298 of 584)	52.4 % (216 of 412)	61.1 % (69 of 113)
Backbone	57.3 % (322 of 562)	56.6 % (111 of 196)	56.8 % (155 of 273)	60.2 % (56 of 93)
Sidechain	49.1 % (310 of 632)	48.5 % (188 of 388)	48.7 % (109 of 224)	65.0 % (13 of 20)
Aromatic	15.6 % (10 of 64)	15.6 % (5 of 32)	16.1 % (5 of 31)	0.0 % (0 of 1)
Methyl	62.5 % (55 of 88)	63.6 % (28 of 44)	61.4 % (27 of 44)

1. HlyIIC-trans (minor)

DNQKALEEQM NSINSVNDKL NKGKGKLSLS MNGNQLKATS SNAGYGISYE DKNWGIFVNG EKVYTFNEKS TVGNISNDIN KLNIKGPYIE IKQI

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	HlyIIC-trans	[U-99% 13C; U-99% 15N]	1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	EDTA	natural abundance	1 mM
4	AEBSF	natural abundance	1 mM
5	sodium azide	natural abundance	0.05 % w/v

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Concentration
6	HlyIIC-trans	[U-99% 15N]	1.0 mM
7	sodium phosphate	natural abundance	20 mM
8	EDTA	natural abundance	1 mM
9	AEBSF	natural abundance	1 mM
10	sodium azide	natural abundance	0.05 % w/v

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Concentration
11	HlyIIC-trans	[U-99% 15N]	1.0 mM
12	sodium phosphate	natural abundance	20 mM
13	EDTA	natural abundance	1 mM
14	AEBSF	natural abundance	1 mM
15	sodium azide	natural abundance	0.05 % w/v

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Concentration
16	HlyIIC-trans	[U-99% 13C; U-99% 15N]	1.0 mM
17	sodium phosphate	natural abundance	20 mM
18	EDTA	natural abundance	1 mM
19	AEBSF	natural abundance	1 mM
20	sodium azide	natural abundance	0.05 % w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 6D53, Strand ID: C Detail

Release date

2013-09-04

Citation

NMR assignments for the cis and trans forms of the hemolysin II C-terminal domain
Kaplan, A.R., Maciejewski, M.W., Olson, R., Alexandrescu, A.T.
Biomol. NMR Assign. (2014), 8, 419-423, PubMed 24234348 , DOI 10.1007/s12104-013-9530-2 , Abstract

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 30737 released on 2020-04-13
    Title Dimeric form of the trans-stabilized Hemolysin II C-terminal domain
  BMRB: 19461 released on 2013-08-27
    Title HlyII-C major cis form
  BMRB: 19463 released on 2013-08-27
    Title p87m-BMRB