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BMRB: 19546

2MFE

Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL2)/RsmE(dimer) 2:1 complex

Authors

Duss, O., Diarra Dit Konte, N., Michel, E., Schubert, M., Allain, F.H. T.

Assembly

RsmZ(SL2)/RsmE(dimer) 2:1 complex

Entity

1. RsmE (polymer, Thiol state: not present), 65 monomers, 7147.134 × 2 Da Detail

MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPH

2. SL2(RsmZ) (polymer, Thiol state: not present), 22 monomers, 7175.277 × 2 Da Detail

GGGCCAUCAA GGACGAUGGU CC

Total weight

28644.82 Da

Max. entity weight

7175.277 Da

Source organism

Pseudomonas fluorescens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 74.7 %, Completeness: 60.0 %, Completeness (bb): 51.8 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	60.0 % (656 of 1093)	62.9 % (364 of 579)	52.0 % (226 of 435)	83.5 % (66 of 79)
Backbone	51.8 % (324 of 626)	48.7 % (129 of 265)	45.2 % (135 of 299)	96.8 % (60 of 62)
Sidechain	74.0 % (389 of 526)	74.8 % (235 of 314)	75.9 % (148 of 195)	35.3 % (6 of 17)
Aromatic	9.1 % (10 of 110)	10.9 % (6 of 55)	9.1 % (4 of 44)	0.0 % (0 of 11)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. RsmE

MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPH

2. SL2(RsmZ)

GGGCCAUCAA GGACGAUGGU CC

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	SL2(RsmZ)	natural abundance	1 mM
3	potassium phosphate	natural abundance	0.05 mM
4	sodium chloride	natural abundance	0.03 mM
5	H2O	natural abundance	95 %
6	D2O	[U-100% 2H]	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 15N]	1 mM
8	SL2(RsmZ)	natural abundance	1 mM
9	potassium phosphate	natural abundance	0.05 mM
10	sodium chloride	natural abundance	0.03 mM
11	D2O	[U-100% 2H]	100 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	SL2(RsmZ)	natural abundance	1 mM
14	potassium phosphate	natural abundance	0.05 mM
15	sodium chloride	natural abundance	0.03 mM
16	H2O	natural abundance	95 %
17	D2O	[U-100% 2H]	5 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 13C; U-100% 15N]	1 mM
19	SL2(RsmZ)	natural abundance	1 mM
20	potassium phosphate	natural abundance	0.05 mM
21	sodium chloride	natural abundance	0.03 mM
22	D2O	[U-100% 2H]	100 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
23	RsmE	[U-100% 15N]	1 mM
24	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
25	potassium phosphate	natural abundance	0.05 mM
26	sodium chloride	natural abundance	0.03 mM
27	H2O	natural abundance	95 %
28	D2O	[U-100% 2H]	5 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
34	RsmE	[U-100% 15N]	1 mM
35	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
36	potassium phosphate	natural abundance	0.05 mM
37	sodium chloride	natural abundance	0.03 mM
38	H2O	natural abundance	95 %
39	D2O	[U-100% 2H]	5 %

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

Chem. Shift Complete2

Sequence coverage: 37.9 %, Completeness: 48.9 %, Completeness (bb): 47.5 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	48.9 % (1069 of 2186)	50.1 % (580 of 1158)	46.2 % (402 of 870)	55.1 % (87 of 158)
Backbone	47.5 % (595 of 1252)	50.0 % (265 of 530)	43.5 % (260 of 598)	56.5 % (70 of 124)
Sidechain	51.2 % (539 of 1052)	50.2 % (315 of 628)	53.1 % (207 of 390)	50.0 % (17 of 34)
Aromatic	46.4 % (102 of 220)	47.3 % (52 of 110)	44.3 % (39 of 88)	50.0 % (11 of 22)
Methyl	59.6 % (112 of 188)	59.6 % (56 of 94)	59.6 % (56 of 94)

1. RsmE

MLILTRKVGE SINIGDDITI TILGVSGQQV RIGINAPKDV AVHREEIYQR IQAGLTAPDK RETPH

2. SL2(RsmZ)

GGGCCAUCAA GGACGAUGGU CC

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	SL2(RsmZ)	natural abundance	1 mM
3	potassium phosphate	natural abundance	0.05 mM
4	sodium chloride	natural abundance	0.03 mM
5	H2O	natural abundance	95 %
6	D2O	[U-100% 2H]	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 15N]	1 mM
8	SL2(RsmZ)	natural abundance	1 mM
9	potassium phosphate	natural abundance	0.05 mM
10	sodium chloride	natural abundance	0.03 mM
11	D2O	[U-100% 2H]	100 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	SL2(RsmZ)	natural abundance	1 mM
14	potassium phosphate	natural abundance	0.05 mM
15	sodium chloride	natural abundance	0.03 mM
16	H2O	natural abundance	95 %
17	D2O	[U-100% 2H]	5 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 13C; U-100% 15N]	1 mM
19	SL2(RsmZ)	natural abundance	1 mM
20	potassium phosphate	natural abundance	0.05 mM
21	sodium chloride	natural abundance	0.03 mM
22	D2O	[U-100% 2H]	100 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
23	RsmE	[U-100% 15N]	1 mM
24	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
25	potassium phosphate	natural abundance	0.05 mM
26	sodium chloride	natural abundance	0.03 mM
27	H2O	natural abundance	95 %
28	D2O	[U-100% 2H]	5 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

Sample #7

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
34	RsmE	[U-100% 15N]	1 mM
35	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
36	potassium phosphate	natural abundance	0.05 mM
37	sodium chloride	natural abundance	0.03 mM
38	H2O	natural abundance	95 %
39	D2O	[U-100% 2H]	5 %

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MFE, Strand ID: A, C Detail

Release date

2014-02-23

Citation

Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition
Duss, O., Michel, E., Diarra Dit Konte, N., Schubert, M., Allain, F.
Nucleic Acids Res. (2014), 42, 5332-5346, PubMed 24561806 , DOI 10.1093/nar/gku141 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 19544 released on 2014-02-23
    Title Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL1)/RsmE(dimer) 2:1 complex
  BMRB: 19547 released on 2014-02-23
    Title Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL3)/RsmE(dimer) 2:1 complex
  BMRB: 19548 released on 2014-02-23
    Title Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL4)/RsmE(dimer) 2:1 complex
  BMRB: 19549 released on 2014-02-23
    Title Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(36-44)/RsmE(dimer) 2:1 complex

Related entities 1. RsmE, : 1 : 7 : 103 entities Detail

Related entities 2. SL2(RsmZ), : 1 : 2 entities Detail

Experiments performed 21 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	SL2(RsmZ)	natural abundance	1 mM
3	potassium phosphate	natural abundance	0.05 mM
4	sodium chloride	natural abundance	0.03 mM
5	H2O	natural abundance	95 %
6	D2O	[U-100% 2H]	5 %

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	SL2(RsmZ)	natural abundance	1 mM
3	potassium phosphate	natural abundance	0.05 mM
4	sodium chloride	natural abundance	0.03 mM
5	H2O	natural abundance	95 %
6	D2O	[U-100% 2H]	5 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	RsmE	[U-100% 15N]	1 mM
2	SL2(RsmZ)	natural abundance	1 mM
3	potassium phosphate	natural abundance	0.05 mM
4	sodium chloride	natural abundance	0.03 mM
5	H2O	natural abundance	95 %
6	D2O	[U-100% 2H]	5 %

4 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 15N]	1 mM
8	SL2(RsmZ)	natural abundance	1 mM
9	potassium phosphate	natural abundance	0.05 mM
10	sodium chloride	natural abundance	0.03 mM
11	D2O	[U-100% 2H]	100 %

5 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 13C; U-100% 15N]	1 mM
19	SL2(RsmZ)	natural abundance	1 mM
20	potassium phosphate	natural abundance	0.05 mM
21	sodium chloride	natural abundance	0.03 mM
22	D2O	[U-100% 2H]	100 %

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	SL2(RsmZ)	natural abundance	1 mM
14	potassium phosphate	natural abundance	0.05 mM
15	sodium chloride	natural abundance	0.03 mM
16	H2O	natural abundance	95 %
17	D2O	[U-100% 2H]	5 %

7 3D 1Fe3Ff NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 13C; U-100% 15N]	1 mM
19	SL2(RsmZ)	natural abundance	1 mM
20	potassium phosphate	natural abundance	0.05 mM
21	sodium chloride	natural abundance	0.03 mM
22	D2O	[U-100% 2H]	100 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
18	RsmE	[U-100% 13C; U-100% 15N]	1 mM
19	SL2(RsmZ)	natural abundance	1 mM
20	potassium phosphate	natural abundance	0.05 mM
21	sodium chloride	natural abundance	0.03 mM
22	D2O	[U-100% 2H]	100 %

9 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	SL2(RsmZ)	natural abundance	1 mM
14	potassium phosphate	natural abundance	0.05 mM
15	sodium chloride	natural abundance	0.03 mM
16	H2O	natural abundance	95 %
17	D2O	[U-100% 2H]	5 %

10 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
12	RsmE	[U-100% 13C; U-100% 15N]	1 mM
13	SL2(RsmZ)	natural abundance	1 mM
14	potassium phosphate	natural abundance	0.05 mM
15	sodium chloride	natural abundance	0.03 mM
16	H2O	natural abundance	95 %
17	D2O	[U-100% 2H]	5 %

11 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
7	RsmE	[U-100% 15N]	1 mM
8	SL2(RsmZ)	natural abundance	1 mM
9	potassium phosphate	natural abundance	0.05 mM
10	sodium chloride	natural abundance	0.03 mM
11	D2O	[U-100% 2H]	100 %

12 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
23	RsmE	[U-100% 15N]	1 mM
24	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
25	potassium phosphate	natural abundance	0.05 mM
26	sodium chloride	natural abundance	0.03 mM
27	H2O	natural abundance	95 %
28	D2O	[U-100% 2H]	5 %

13 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
34	RsmE	[U-100% 15N]	1 mM
35	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
36	potassium phosphate	natural abundance	0.05 mM
37	sodium chloride	natural abundance	0.03 mM
38	H2O	natural abundance	95 %
39	D2O	[U-100% 2H]	5 %

14 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

15 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

16 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

17 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

18 3D 1Fe3Ff NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

19 3D 1Fe3Ff NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

20 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
29	RsmE	[U-100% 15N]	1 mM
30	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Ade/Ura RNA	1 mM
31	potassium phosphate	natural abundance	0.05 mM
32	sodium chloride	natural abundance	0.03 mM
33	D2O	[U-100% 2H]	100 %

21 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 313 K, pH 7.2

#	Name	Isotope labeling	Concentration
40	RsmE	[U-100% 15N]	1 mM
41	SL2(RsmZ)	[U-100% 13C; U-100% 15N]-Cyt/Gua RNA	1 mM
42	potassium phosphate	natural abundance	0.05 mM
43	sodium chloride	natural abundance	0.03 mM
44	D2O	[U-100% 2H]	100 %

nullKeywords bacterial protein, CsrA, CsrB, homo-dimeric protein, messenger RNA, modulation of binding affinity, molecular mimicry, NMR, non-coding RNA, protein sequestration, Pseudomonas aeruginosa, RNA-Binding Proteins, RsmA, RsmE, RsmZ, sRNA, translation activation, translation repressor protein

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Found 1 Document (1.124 seconds)

BMRB: 19546

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Sample #8

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 4 entries Detail

Related entities 1. RsmE, : 1 : 7 : 103 entities Detail

Related entities 2. SL2(RsmZ), : 1 : 2 entities Detail

Experiments performed 21 experiments Detail

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Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample