BMRB: 25457

Cullin3 - BTB interface: a novel target for stapled peptides

Authors

Russo, L., Palmieri, M., Malgieri, G.

Assembly

Cullin3 - BTB interface

Entity

1. Cullin3 - BTB interface (polymer, Thiol state: not present), 20 monomers, 2442.786 Da Detail

NSGLSFEEXY RNXYTMVLHK

Formula weight

2442.786 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 87.8 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.8 % (101 of 115)	87.8 % (101 of 115)
Backbone	100.0 % (37 of 37)	100.0 % (37 of 37)
Sidechain	82.1 % (64 of 78)	82.1 % (64 of 78)
Aromatic	53.3 % (8 of 15)	53.3 % (8 of 15)
Methyl	71.4 % (5 of 7)	71.4 % (5 of 7)

1. entity

NSGLSFEEXY RNXYTMVLHK

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 3

#	Name	Isotope labeling	Type	Concentration
1	Cul349-68LA	natural abundance		1 mM
2	trifluoroethanol	natural abundance		0.0 ~ 0.0 %
3	water	natural abundance		0.0 ~ 0.0 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2MYM, Strand ID: A Detail

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Release date

2015-04-19

Citation

Cullin3-BTB interface: a novel target for stapled peptides
de Paola, I., Pirone, L., Palmieri, M., Balasco, N., Esposito, L., Russo, L., Mazz, D., Di Marcotullio, L., Di Gaetano, S., Malgieri, G., Vitagliano, L., Pedone, E., Zaccaro, L.
PLoS One (2015), 10, e0121149-e0121149, PubMed 25848797 , DOI 10.1371/journal.pone.0121149 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Cullin3 - BTB interface, : 1 : 18 : 23 entities Detail

Interaction partners 1. Cullin3 - BTB interface, : 16 interactors Detail

Experiments performed 2 experiments Detail