NMR structure of the RRM3 domain of Gbp2
GSHIDETAAK FTEGVNPGGD RNCFIYCSNL PFSTARSDLF DLFGPIGKIN NAELKPQENG QPTGVAVVEY ENLVDADFCI QKLNNYNYGG CSLQISYARR D
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.2 % (1038 of 1138) | 94.7 % (559 of 590) | 84.7 % (371 of 438) | 98.2 % (108 of 110) |
Backbone | 96.3 % (574 of 596) | 97.6 % (202 of 207) | 94.9 % (278 of 293) | 97.9 % (94 of 96) |
Sidechain | 87.7 % (555 of 633) | 93.2 % (357 of 383) | 78.0 % (184 of 236) | 100.0 % (14 of 14) |
Aromatic | 46.2 % (48 of 104) | 92.3 % (48 of 52) | 0.0 % (0 of 52) | |
Methyl | 100.0 % (94 of 94) | 100.0 % (47 of 47) | 100.0 % (47 of 47) |
1. RRM3
GSHIDETAAK FTEGVNPGGD RNCFIYCSNL PFSTARSDLF DLFGPIGKIN NAELKPQENG QPTGVAVVEY ENLVDADFCI QKLNNYNYGG CSLQISYARR DSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RRM3 | [U-100% 13C; U-100% 15N] | 0.8 mM | |
2 | potassium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 25 mM | |
4 | DTT | natural abundance | 0.1 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RRM3 | [U-100% 13C; U-100% 15N] | 0.8 mM | |
8 | potassium phosphate | natural abundance | 25 mM | |
9 | sodium chloride | natural abundance | 25 mM | |
10 | DTT | natural abundance | 0.1 mM | |
11 | D2O | natural abundance | 100 % |