NMR structure of the RRM3 domain of Hrb1
GSVNEEARKF TENVVGGGER NRLIYCSNLP FSTAKSDLYD LFETIGKVNN AELRYDSKGA PTGIAVVEYD NVDDADVCIE RLNNYNYGGC DLDISYAKRL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.2 % (962 of 1116) | 95.8 % (552 of 576) | 70.4 % (304 of 432) | 98.1 % (106 of 108) |
Backbone | 84.6 % (504 of 596) | 97.6 % (202 of 207) | 70.8 % (206 of 291) | 98.0 % (96 of 98) |
Sidechain | 89.9 % (549 of 611) | 94.9 % (350 of 369) | 81.5 % (189 of 232) | 100.0 % (10 of 10) |
Aromatic | 51.2 % (44 of 86) | 100.0 % (43 of 43) | 2.3 % (1 of 43) | |
Methyl | 100.0 % (106 of 106) | 100.0 % (53 of 53) | 100.0 % (53 of 53) |
1. RRM3
GSVNEEARKF TENVVGGGER NRLIYCSNLP FSTAKSDLYD LFETIGKVNN AELRYDSKGA PTGIAVVEYD NVDDADVCIE RLNNYNYGGC DLDISYAKRLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RRM3 | [U-100% 13C; U-100% 15N] | 0.8 mM | |
2 | potassium phosphate | natural abundance | 25 mM | |
3 | sodium chloride | natural abundance | 25 mM | |
4 | DTT | natural abundance | 0.1 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RRM3 | [U-100% 13C; U-100% 15N] | 0.8 mM | |
8 | potassium phosphate | natural abundance | 25 mM | |
9 | sodium chloride | natural abundance | 25 mM | |
10 | DTT | natural abundance | 0.1 mM | |
11 | D2O | natural abundance | 100 % |