BMRB: 25681

FBP28 WW E454Y

Authors

Medina, J., Macias, M., Martin-Malpartida, P., Scheraga, H.A.

Assembly

E454Y

Entity

1. E454Y (polymer, Thiol state: not present), 37 monomers, 4393.728 Da Detail

GATAVSEWTE YKTADGKTYY YNNRTLYSTW EKPQELK

Formula weight

4393.728 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 44.7 %, Completeness (bb): 34.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	44.7 % (199 of 445)	73.5 % (169 of 230)	14.9 % (26 of 174)	9.8 % (4 of 41)
Backbone	34.1 % (75 of 220)	76.0 % (57 of 75)	12.8 % (14 of 109)	11.1 % (4 of 36)
Sidechain	48.8 % (127 of 260)	72.3 % (112 of 155)	15.0 % (15 of 100)	0.0 % (0 of 5)
Aromatic	32.8 % (21 of 64)	59.4 % (19 of 32)	6.7 % (2 of 30)	0.0 % (0 of 2)
Methyl	63.3 % (19 of 30)	93.3 % (14 of 15)	33.3 % (5 of 15)

1. entity

GATAVSEWTE YKTADGKTYY YNNRTLYSTW EKPQELK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		500.0 ~ 1000.0 uM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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Calculated from 20 models in PDB: 2N4U, Strand ID: A Detail

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Release date

2015-10-18

Citation

Preventing fibril formation of a protein by selective mutation
Maisuradze, G.G., Medina, J., Kachlishvili, K., Krupa, P., Mozolewska, M., Martin-Malpartida, P., Maisuradze, L., Macias, M., Scheraga, H.A.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 13549-13554, PubMed 26483482 , DOI 10.1073/pnas.1518298112 , Abstract

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. E454Y, : 1 : 17 : 118 entities Detail

Interaction partners 1. E454Y, : 20 interactors Detail

Experiments performed 2 experiments Detail