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BMRB: 25871

2N8Z

Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Authors

Ilc, G., Drmota Prebil, S., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.

Assembly

Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Entity

1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1 (polymer), 153 monomers, 16973.65 Da Detail

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL

Formula weight

16973.65 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 91.0 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1561 of 1715)	97.3 % (862 of 886)	81.1 % (546 of 673)	98.1 % (153 of 156)
Backbone	85.6 % (772 of 902)	97.7 % (302 of 309)	73.2 % (328 of 448)	97.9 % (142 of 145)
Sidechain	97.2 % (928 of 955)	97.1 % (560 of 577)	97.3 % (357 of 367)	100.0 % (11 of 11)
Aromatic	94.9 % (148 of 156)	94.9 % (74 of 78)	94.9 % (74 of 78)
Methyl	98.6 % (138 of 140)	98.6 % (69 of 70)	98.6 % (69 of 70)

1. Calmodulin-Like Domain of alpha-actinin 1

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
1	Calmodulin-Like Domain of alpha-actinin 1	[U-100% 13C; U-100% 15N]	1 mM
2	HEPES	natural abundance	20 mM
3	Sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1.5 mM
5	EGTA	natural abundance	0.05 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: -0.18 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: -0.18 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N8Z, Strand ID: A Detail

Release date

2016-06-26

Citation

Structure and calcium-binding studies of calmodulin-like domain of human non-muscle α-actinin-1
Drmota Prebil, S., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Ilc, G., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.
Sci. Rep. (2016), 6, 27383-27383, PubMed 27272015 , DOI 10.1038/srep27383 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25870 released on 2016-06-26
Title Holo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Related entities 1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1, : 1 : 1 : 6 : 74 entities Detail

Interaction partners 1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1, : 90 interactors Detail

More details for 90 interactors identified by 46 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC