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BMRB: 25870

2N8Y

Holo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Authors

Drmota Prebil, S.D., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Ilc, G., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.

Assembly

Holo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Entity

1. Calmodulin-Like Domain of alpha-actinin 1 (polymer), 153 monomers, 16973.65 Da Detail

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL

2. CALCIUM ION (non-polymer), 40.078 Da

Total weight

17013.729 Da

Max. entity weight

16973.65 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 91.9 %, Completeness (bb): 85.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.9 % (1576 of 1715)	98.5 % (873 of 886)	81.7 % (550 of 673)	98.1 % (153 of 156)
Backbone	85.5 % (771 of 902)	98.4 % (304 of 309)	72.5 % (325 of 448)	97.9 % (142 of 145)
Sidechain	99.2 % (947 of 955)	98.6 % (569 of 577)	100.0 % (367 of 367)	100.0 % (11 of 11)
Aromatic	100.0 % (156 of 156)	100.0 % (78 of 78)	100.0 % (78 of 78)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. Calmodulin-Like Domain of alpha-actinin 1

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
1	Calmodulin-Like Domain of alpha-actinin 1	[U-100% 13C; U-100% 15N]	1 mM
2	HEPES	natural abundance	20 mM
3	Sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1.5 mM
5	EGTA	natural abundance	0.05 mM
6	Calcium chloride	natural abundance	20 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: -0.05 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	external	direct	1.0
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	ammonium hydroxide	nitrogen	external	direct	1.0

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N8Y, Strand ID: A Detail

Release date

2016-06-26

Citation

Structure and calcium-binding studies of calmodulin-like domain of human non-muscle α-actinin-1
Drmota Prebil, S.D., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Ilc, G., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.
Sci. Rep. (2016), 6, 27383-27383, PubMed 27272015 , DOI 10.1038/srep27383 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25871 released on 2016-06-26
Title Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1

Related entities 1. Calmodulin-Like Domain of alpha-actinin 1, : 1 : 1 : 6 : 74 entities Detail

Interaction partners 1. Calmodulin-Like Domain of alpha-actinin 1, : 90 interactors Detail

More details for 90 interactors identified by 46 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC