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BMRB: 25899

2N9B

Solution NMR Structure of Antiparallel Myosin-10:GCN4 Tandem Coiled-Coil

Authors

Vavra, K.C., Xia, Y., Rock, R.S.

Assembly

RockCC

Entity

1. RockCC (polymer, Thiol state: not present), 69 monomers, 8186.175 × 2 Da Detail

GSHENKQVEE ILRLEKEIED LQRMKERQEL SLTEASLQKL QLEDKVEELL SKNYHLENEV ARLKKLVGE

Total weight

16372.35 Da

Max. entity weight

8186.175 Da

Source organism

Bos taurus , Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.8 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.8 % (810 of 854)	94.1 % (429 of 456)	95.6 % (307 of 321)	96.1 % (74 of 77)
Backbone	96.9 % (401 of 414)	95.7 % (134 of 140)	98.0 % (201 of 205)	95.7 % (66 of 69)
Sidechain	93.7 % (475 of 507)	93.4 % (295 of 316)	94.0 % (172 of 183)	100.0 % (8 of 8)
Aromatic	75.0 % (12 of 16)	75.0 % (6 of 8)	75.0 % (6 of 8)
Methyl	84.1 % (69 of 82)	82.9 % (34 of 41)	85.4 % (35 of 41)

1. entity

GSHENKQVEE ILRLEKEIED LQRMKERQEL SLTEASLQKL QLEDKVEELL SKNYHLENEV ARLKKLVGE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 35 K, pH 6.5, Details Sodium azide added for sample stability

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	1.1 mM
2	potassium phosphate	natural abundance	100 mM
3	EDTA	natural abundance	1 mM
4	sodium azide	natural abundance	0.03 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2N9B, Strand ID: A, B Detail

Release date

2016-06-07

Citation 1

Antiparallel coiled-coil-mediated dimerization of myosin X
Lu, Q., Ye, F., Wei, Z., Wen, Z., Zhang, M.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 17388-17393, PubMed 23012428 , DOI 10.1073/pnas.1208642109 , Abstract

Show more 4 citaions

Citation 2

X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil
O'Shea, E.K., Klemm, J.D., Kim, P.S., Alber, T.
Science (1991), 254, 539-544, PubMed 1948029 , Abstract

Citation 3

Competition between Coiled-Coil Structures and the Impact on Myosin-10 Bundle Selection
Vavra, K.C., Xia, Y., Rock, R.S.
Biophys. J. (2016), 110, 2517-2527, PubMed 27276269 , DOI 10.1016/j.bpj.2016.04.048 , Abstract

Citation 4

A myosin motor that selects bundled actin for motility
Nagy, S., Ricca, B.L., Norstrom, M.F., Courson, D.F., Brawley, C.M., Smithback, P.A., Rock, R.S.
Proc. Natl. Acad. Sci. U. S. A. (2008), 105, 9616-9620, PubMed 18599451 , DOI 10.1073/pnas.0802592105 , Abstract

Citation 5

Spare the rod, spoil the regulation: necessity for a myosin rod
Trybus, K.M., Freyzon, Y., Faust, L.Z., Sweeney, H.
Proc. Natl. Acad. Sci. U. S. A. (1997), 94, 48-52, PubMed 8990159 , Abstract

Hide non-primary 4 citaions

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 18614 released on 2012-09-23
    Title NMR solution structure of Myo10 anti-CC
  Swiss-Prot: Q9HD67 released on 2001-12-05
    Title MYO10_HUMAN Entity Unconventional myosin-X
  Swiss-Prot: P03069 released on 1986-07-21
    Title GCN4_YEAST Entity General control protein GCN4

Related entities 1. RockCC, : 1 : 19 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC