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BMRB: 25930

2NA7

Transmembrane domain of human Fas/CD95 death receptor

Authors

Fu, Q., Chou, J.J.

Assembly

Transmembrane domain of human Fas/CD95 death receptor

Entity

1. Transmembrane domain of human Fas/CD95 death receptor (polymer, Thiol state: not present), 28 monomers, 3301.019 × 3 Da Detail

RSNLGWLSLL LLPIPLIVWV KRKEVQKT

Total weight

9903.057 Da

Max. entity weight

3301.019 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 27.8 %, Completeness (bb): 62.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	27.8 % (103 of 371)	13.8 % (27 of 195)	35.6 % (52 of 146)	80.0 % (24 of 30)
Backbone	62.8 % (103 of 164)	49.1 % (27 of 55)	62.7 % (52 of 83)	92.3 % (24 of 26)
Sidechain	0.4 % (1 of 234)	0.0 % (0 of 140)	1.1 % (1 of 90)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 24)	0.0 % (0 of 12)	0.0 % (0 of 10)	0.0 % (0 of 2)
Methyl	0.0 % (0 of 50)	0.0 % (0 of 25)	0.0 % (0 of 25)

1. Human Fas Transmembrane Domain

RSNLGWLSLL LLPIPLIVWV KRKEVQKT

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Human Fas Transmembrane Domain	[U-100% 13C; U-100% 15N]	1 (±0.05) mM
2	DMPC	[U-100% 2H] acyl chains	60 (±1.0) mM
3	DHPC	[U-100% 2H] acyl chains	120 (±5.0) mM
4	sodium phosphate	natural abundance	20 (±1.0) mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
7	Human Fas Transmembrane Domain	[U-100% 13C; U-100% 15N; U-85% 2H]	1 (±0.05) mM
8	DMPC	natural abundance	60 (±1.0) mM
9	DHPC	natural abundance	120 (±5.0) mM
10	sodium phosphate	natural abundance	20 (±1.0) mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
13	Human Fas Transmembrane Domain	[U-100% 15N; U-100% 2H]	0.5 (±0.05) mM
14	Human Fas Transmembrane Domain	[U-15% 13C]	0.5 (±0.05) mM
15	DMPC	[U-100% 2H] acyl chains	60 (±1.0) mM
16	DHPC	[U-100% 2H] acyl chains	120 (±5.0) mM
17	sodium phosphate	natural abundance	20 (±1.0) mM
18	H2O	natural abundance	95 %
19	D2O	natural abundance	5 %

Protein Blocks Logo

Calculated from 15 models in PDB: 2NA7, Strand ID: A, B, C Detail

Release date

2016-01-24

Citation

Structural Basis and Functional Role of Intramembrane Trimerization of the Fas/CD95 Death Receptor
Fu, Q., Fu, T., Cruz, A.C., Sengupta, P., Thomas, S.K., Wang, S., Siegel, R.M., Wu, H., Chou, J.J.
Mol. Cell (2016), 61, 602-613, PubMed 26853147 , DOI 10.1016/j.molcel.2016.01.009 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25929 released on 2016-01-24
Title Transmembrane domain of mouse Fas/CD95 death receptor

Related entities 1. Transmembrane domain of human Fas/CD95 death receptor, : 1 : 2 : 1 entities Detail

Interaction partners 1. Transmembrane domain of human Fas/CD95 death receptor, : 45 interactors Detail

More details for 45 interactors identified by 14 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N TROSY HSQC