Solution structure of the K domain of EMCV IRES
Polymer type: polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 55.3 % (343 of 620) | 50.0 % (176 of 352) | 62.3 % (167 of 268) |
Suger, PO4 | 48.2 % (212 of 440) | 45.8 % (110 of 240) | 51.0 % (102 of 200) |
Nucleobase | 72.8 % (131 of 180) | 58.9 % (66 of 112) | 95.6 % (65 of 68) |
Aromatic | 81.9 % (131 of 160) | 71.7 % (66 of 92) | 95.6 % (65 of 68) |
1. RNA (40-MER)
GGGCUCGGUG CACAUGCUUU ACAUGUGUUU AGUCGAGCCCSolvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (40-MER) | natural abundance | RNA | 0.5 mM |
2 | potassium phosphate | natural abundance | buffer | 10 mM |
3 | sodium chloride | natural abundance | salt | 10 mM |
4 | D2O | [U-2H] | solvent | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | RNA (40-MER) | [U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt | RNA | 0.5 mM |
11 | potassium phosphate | natural abundance | buffer | 10 mM |
12 | sodium chloride | natural abundance | salt | 10 mM |
13 | D2O | [U-2H] | solvent | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | RNA (40-MER) | [U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura | RNA | 0.5 mM |
15 | potassium phosphate | natural abundance | buffer | 10 mM |
16 | sodium chloride | natural abundance | salt | 10 mM |
17 | D2O | [U-2H] | solvent | 100 % |