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BMRB: 27852

6F8L

NMR resonance assignments for the GSPII-B domain of the traffic ATPase PilF from Thermus thermophilus in the apo and c-di-GMP-bound states

Authors

Neissner, K., Keller, H., Hacker, C., Durchardt-Ferner, E., Woehnert, J.

Assembly

apo tate PilF159-302

Entity

1. apo tate PilF159-302 (polymer, Thiol state: all free), 146 monomers, 16249.39 Da Detail

GSSGEGQKDL KLGELLLQKG WISREALEEA LVEQEKTGDL LGRILVRKGL PEEALYRALA EQKGLEFLES TEGIVPDPSA ALLLLRSDAL RYGAVPIGFQ NGEVEVVLSD PRHKEAVAQL LNRPARFYLA LPQAWEELFR RAYPQK

Formula weight

16249.39 Da

Source organism

Thermus thermophilus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.7 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.7 % (1666 of 1722)	95.9 % (866 of 903)	97.5 % (652 of 669)	98.7 % (148 of 150)
Backbone	98.7 % (849 of 860)	99.0 % (294 of 297)	98.6 % (419 of 425)	98.6 % (136 of 138)
Sidechain	95.4 % (949 of 995)	94.4 % (572 of 606)	96.8 % (365 of 377)	100.0 % (12 of 12)
Aromatic	80.0 % (80 of 100)	80.0 % (40 of 50)	79.2 % (38 of 48)	100.0 % (2 of 2)
Methyl	96.8 % (180 of 186)	94.6 % (88 of 93)	98.9 % (92 of 93)

1. PilF159-302

GSSGEGQKDL KLGELLLQKG WISREALEEA LVEQEKTGDL LGRILVRKGL PEEALYRALA EQKGLEFLES TEGIVPDPSA ALLLLRSDAL RYGAVPIGFQ NGEVEVVLSD PRHKEAVAQL LNRPARFYLA LPQAWEELFR RAYPQK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	PilF159-302	[U-13C; U-15N]	523 uM
2	D2O	natural abundance	10 %
3	DSS	natural abundance	200 uM
4	Bis-TRIS	natural abundance	50 mM
5	beta-mercaptoethanol	natural abundance	1 %
6	sodium chloride	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Release date

2019-03-25

Citation

NMR resonance assignments for the GSPII-B domain of the traffic ATPase PilF from Thermus thermophilus in the apo and the c-di-GMP-bound state
Neissner, K., Keller, H., Duchardt-Ferner, E., Hacker, C., Kruse, K., Averhoff, B., Wohnert, J.
Biomol. NMR Assign. (2019), 13, 383-390, PubMed 31432400 , DOI 10.1007/s12104-019-09911-z , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27853 released on 2019-03-25
Title NMR resonance assignments for the GSPII-B domain of the traffic ATPase PilF from Thermus thermophilus in the apo and c-di-GMP-bound states

Related entities 1. apo tate PilF159-302, : 1 : 3 : 1 : 2 : 2 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC