NMR resonance assignments of the hazelnut allergen isoform Cor a 1.0404
GVFSYEDEAT SVIPPARLFK SFVLDADNLI PKVAPQHFTS AENLEGNGGP GTIKKITFAE GNEFKYMKHK VEEIDHANFK YCYSIIEGGP LGHTLEKIPY EIKMAAAPHG GGSILKITSK YHTKGNASIN EEEIKAGKEK AAGLFKAVEA YLLAHPDAYC
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.4 % (1616 of 1848) | 86.0 % (824 of 958) | 88.2 % (645 of 731) | 92.5 % (147 of 159) |
Backbone | 91.8 % (865 of 942) | 90.2 % (294 of 326) | 92.9 % (432 of 465) | 92.1 % (139 of 151) |
Sidechain | 84.5 % (888 of 1051) | 83.9 % (530 of 632) | 85.2 % (350 of 411) | 100.0 % (8 of 8) |
Aromatic | 67.4 % (116 of 172) | 68.6 % (59 of 86) | 66.3 % (57 of 86) | |
Methyl | 97.0 % (161 of 166) | 96.4 % (80 of 83) | 97.6 % (81 of 83) |
1. Cor a 1.0404
GVFSYEDEAT SVIPPARLFK SFVLDADNLI PKVAPQHFTS AENLEGNGGP GTIKKITFAE GNEFKYMKHK VEEIDHANFK YCYSIIEGGP LGHTLEKIPY EIKMAAAPHG GGSILKITSK YHTKGNASIN EEEIKAGKEK AAGLFKAVEA YLLAHPDAYCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cor a 1.0404 | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | DTT | natural abundance | 2 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | Cor a 1.0404 | [U-99% 15N] | 0.5 mM | |
5 | sodium phosphate | natural abundance | 20 mM | |
6 | DTT | natural abundance | 2 mM |