Solution structure of the de novo miniprotein EEHE_02
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.2 % (311 of 383) | 94.9 % (188 of 198) | 59.2 % (87 of 147) | 94.7 % (36 of 38) |
Backbone | 76.4 % (168 of 220) | 96.1 % (73 of 76) | 56.5 % (61 of 108) | 94.4 % (34 of 36) |
Sidechain | 86.3 % (170 of 197) | 94.3 % (115 of 122) | 72.6 % (53 of 73) | 100.0 % (2 of 2) |
Aromatic | 33.3 % (4 of 12) | 66.7 % (4 of 6) | 0.0 % (0 of 6) | |
Methyl | 100.0 % (32 of 32) | 100.0 % (16 of 16) | 100.0 % (16 of 16) |
1. W37
APCECDVNGE TYTVSSSEEC ERLCRKLGVT NCRVHCGSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 4.8, Details 1 mM [U-99% 15N] W37, 50 mM sodium chloride, 25 mM sodium acetate, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | W37 | [U-99% 15N] | 1 (±0.2) mM | |
2 | sodium acetate | natural abundance | 25 (±1.0) mM | |
3 | sodium chloride | natural abundance | 50 (±1.0) mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 4.8, Details 1 mM [U-99% 15N] W37, 50 mM sodium chloride, 25 mM sodium acetate, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | W37 | [U-99% 15N] | 1 mM | |
7 | sodium acetate | natural abundance | 25 (±1.0) mM | |
8 | sodium chloride | natural abundance | 50 (±1.0) mM | |
9 | D2O | natural abundance | 100 % |