BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	79.7 % (890 of 1117)	69.3 % (413 of 596)	95.8 % (388 of 405)	76.7 % (89 of 116)
Backbone	97.8 % (532 of 544)	97.8 % (178 of 182)	97.4 % (267 of 274)	98.9 % (87 of 88)
Sidechain	67.4 % (447 of 663)	56.8 % (235 of 414)	95.0 % (210 of 221)	7.1 % (2 of 28)
Aromatic	81.6 % (31 of 38)	84.2 % (16 of 19)	78.9 % (15 of 19)
Methyl	96.7 % (87 of 90)	93.3 % (42 of 45)	100.0 % (45 of 45)

1. entity 1

NNPLQQQSSQ NTVPNVLNQI NQIFSPEEQR SLLQEAIETC KNFEKTQLGS TMTEPVKQSF IRKYINQKAL RKIQALRDVK NNNNANNNGS NL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5 (±0.05), Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM EDTA, 0.1 mM PMSF, 5 mM DTT, 500 uM [U-15N] Med15 ABD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	EDTA	natural abundance	0.1 mM
3	Med15 ABD2	[U-15N]	500 uM
4	PMSF	natural abundance	0.1 mM
5	sodium chloride	natural abundance	150 mM
6	sodium phosphate	natural abundance	20 mM

Sample #2

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5 (±0.05), Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM EDTA, 0.1 mM PMSF, 5 mM DTT, 500 uM [U-13C; U-15N] Med15 ABD2, 100% D2O

#	Name	Isotope labeling	Concentration
13	DTT	natural abundance	5 mM
14	EDTA	natural abundance	0.1 mM
15	Med15 ABD2	[U-13C; U-15N]	500 uM
16	PMSF	natural abundance	0.1 mM
17	sodium chloride	natural abundance	150 mM
18	sodium phosphate	natural abundance	20 mM

Sample #4

#	Name	Isotope labeling	Concentration
19	C12E6/Hexanol	natural abundance	10 %
20	DTT	natural abundance	5 mM
21	EDTA	natural abundance	0.1 mM
22	Med15 ABD2	[U-15N]	2.6 mM
23	PMSF	natural abundance	0.1 mM
24	sodium chloride	natural abundance	150 mM
25	sodium phosphate	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6ALY, Strand ID: A Detail

Release date

2017-10-02

Citation

Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex
Tuttle, L.M., Pachecko, D., Warfield, L., Luo, J., Ranish, J., Hahn, S., Klevit, R.E.
Cell Rep. (2018), 22, 3251-3264, PubMed 29562181 , DOI 10.1016/j.celrep.2018.02.097 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27207 released on 2017-08-06
Title Gcn4 tAD 1-134

Related entities 1. Mediator of RNA polymerase II transcription subunit 15, : 1 : 2 : 7 entities Detail

Interaction partners 1. Mediator of RNA polymerase II transcription subunit 15, : 37 interactors Detail

More details for 37 interactors identified by 9 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5 (±0.05), Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM EDTA, 0.1 mM PMSF, 5 mM DTT, 500 uM [U-15N] Med15 ABD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	EDTA	natural abundance	0.1 mM
3	Med15 ABD2	[U-15N]	500 uM
4	PMSF	natural abundance	0.1 mM
5	sodium chloride	natural abundance	150 mM
6	sodium phosphate	natural abundance	20 mM

2 3D HNCO

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

3 3D HNCA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

4 3D HN(CO)CA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

5 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

6 3D HNCACB

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

7 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

8 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5 (±0.05), Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM EDTA, 0.1 mM PMSF, 5 mM DTT, 500 uM [U-13C; U-15N] Med15 ABD2, 100% D2O

#	Name	Isotope labeling	Concentration
13	DTT	natural abundance	5 mM
14	EDTA	natural abundance	0.1 mM
15	Med15 ABD2	[U-13C; U-15N]	500 uM
16	PMSF	natural abundance	0.1 mM
17	sodium chloride	natural abundance	150 mM
18	sodium phosphate	natural abundance	20 mM

9 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

11 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

12 3D 1H-15N TOCSY

Instrument

Bruker AvanceIII - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	EDTA	natural abundance	0.1 mM
3	Med15 ABD2	[U-15N]	500 uM
4	PMSF	natural abundance	0.1 mM
5	sodium chloride	natural abundance	150 mM
6	sodium phosphate	natural abundance	20 mM

13 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	EDTA	natural abundance	0.1 mM
3	Med15 ABD2	[U-15N]	500 uM
4	PMSF	natural abundance	0.1 mM
5	sodium chloride	natural abundance	150 mM
6	sodium phosphate	natural abundance	20 mM

14 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

15 3D C(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DTT	natural abundance	5 mM
8	EDTA	natural abundance	0.1 mM
9	Med15 ABD2	[U-13C; U-15N]	500 uM
10	PMSF	natural abundance	0.1 mM
11	sodium chloride	natural abundance	150 mM
12	sodium phosphate	natural abundance	20 mM

16 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
13	DTT	natural abundance	5 mM
14	EDTA	natural abundance	0.1 mM
15	Med15 ABD2	[U-13C; U-15N]	500 uM
16	PMSF	natural abundance	0.1 mM
17	sodium chloride	natural abundance	150 mM
18	sodium phosphate	natural abundance	20 mM

17 3D HCCH-COSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
13	DTT	natural abundance	5 mM
14	EDTA	natural abundance	0.1 mM
15	Med15 ABD2	[U-13C; U-15N]	500 uM
16	PMSF	natural abundance	0.1 mM
17	sodium chloride	natural abundance	150 mM
18	sodium phosphate	natural abundance	20 mM

18 2D 1H-15N HSQC IPAP

Instrument

Bruker AvanceIII - 800 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5 (±0.05), Details 20 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM EDTA, 0.1 mM PMSF, 5 mM DTT, 10 % C12E6/Hexanol, 2.6 mM [U-15N] Med15 ABD2, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
19	C12E6/Hexanol	natural abundance	10 %
20	DTT	natural abundance	5 mM
21	EDTA	natural abundance	0.1 mM
22	Med15 ABD2	[U-15N]	2.6 mM
23	PMSF	natural abundance	0.1 mM
24	sodium chloride	natural abundance	150 mM
25	sodium phosphate	natural abundance	20 mM

19 2D 1H-15N HSQC IPAP

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DTT	natural abundance	5 mM
2	EDTA	natural abundance	0.1 mM
3	Med15 ABD2	[U-15N]	500 uM
4	PMSF	natural abundance	0.1 mM
5	sodium chloride	natural abundance	150 mM
6	sodium phosphate	natural abundance	20 mM

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_30330_6aly.nef
Input source #2: Coordindates	6aly.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---280-----290-------300-------310-------320-------330-------340-------350-------360--------
NNPLQQQSSQNTVPNVLNQINQIFSPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNNANNNGSNL
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NNPLQQQSSQNTVPNVLNQINQIFSPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNNANNNGSNL
--------10--------20--------30--------40--------50--------60--------70--------80--------90--

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	92	0	0	100.0

Content subtype: combined_30330_6aly.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	875		100.0 (chain: A, length: 92)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	116 (1)	noe	65.2 (chain: A, length: 92)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	24 (1)	noe	13.0 (chain: A, length: 92)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	102 (102)	.	59.8 (chain: A, length: 92)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	70 (70)	.	76.1 (chain: A, length: 92)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 379
  - ¹H chemical shifts: 409
  - ¹⁵N chemical shifts: 87
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	405	379	93.6
¹H chemical shifts	596	380	63.8
¹⁵N chemical shifts	120	87	72.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	184	176	95.7
¹H chemical shifts	182	178	97.8
¹⁵N chemical shifts	88	87	98.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	221	203	91.9
¹H chemical shifts	414	202	48.8
¹⁵N chemical shifts	32	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	46	46	100.0
¹H chemical shifts	46	42	91.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	19	12	63.2
¹H chemical shifts	19	12	63.2

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 65.2%
- Total number of restraints: 116
- Weight of restraints: 1.0 (116)
- Potential type of restraints: square-well-parabolic (116)
- Range of distance target values: 3.4, 4.55 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 13.0%
  - Total number of restraints: 24
  - Weight of restraints: 1.0 (24)
  - Potential type of restraints: square-well-parabolic (24)
  - Range of distance target values: 4.05, 4.3 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 59.8%
- Total number of restraints: 102
- Total number of restraints per polymer type: 102
- Weight of restraints: 1.0 (102)
- Potential type of restraints: square-well-parabolic (102)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 76.1%

---280-----290-------300-------310-------320-------330-------340-------350-------360--------
NNPLQQQSSQNTVPNVLNQINQIFSPEEQRSLLQEAIETCKNFEKTQLGSTMTEPVKQSFIRKYINQKALRKIQALRDVKNNNNANNNGSNL
   ||||||| ||  |||||||||| |||||| ||| || || ||||||||||| |||| |||| |||| || |||| |||   ||  ||||
...LQQQSSQ.TV..VLNQINQIFS.EEQRSL.QEA.ET.KN.EKTQLGSTMTE.VKQS.IRKY.NQKA.RK.QALR.VKN...AN..GSNL

Total number of restraints: 70
Potential type of restraints: undefined (70)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords ABD, Gal11, Helical, MED15, Mediator, TRANSCRIPTION, Transcription Activation, yeast

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BMRB: 30330

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Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Mediator of RNA polymerase II transcription subunit 15, : 1 : 2 : 7 entities Detail

Interaction partners 1. Mediator of RNA polymerase II transcription subunit 15, : 37 interactors Detail

Experiments performed 19 experiments Detail

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NMR combined restraints 6 contents Detail