BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	31.5 % (278 of 883)	17.1 % (85 of 497)	40.4 % (126 of 312)	90.5 % (67 of 74)
Backbone	64.5 % (263 of 408)	52.2 % (71 of 136)	61.3 % (125 of 204)	98.5 % (67 of 68)
Sidechain	13.8 % (75 of 543)	3.9 % (14 of 361)	34.7 % (61 of 176)	0.0 % (0 of 6)
Methyl	13.6 % (3 of 22)	0.0 % (0 of 11)	27.3 % (3 of 11)

1. entity 1

KQQEEEAERL RRIQEEMEKE RKRREEDEQR RRKEEEERRM KLEMEAKRKQ EEEERKKRED DEKRIQAE

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

Sample #3

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13-; U-2H I13C MT-DOTA-Lu/Tm, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	entity_1	[U-15N; U-13C; U-2H]		1 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6OBI, Strand ID: A Detail

Release date

2019-06-03

Citation

Remarkable Rigidity of the Single α-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy
Barnes, C.A., Shen, Y., Ying, J., Takagi, Y., Torchia, D.A., Sellers, J.R., Bax, A.
J. Am. Chem. Soc. (2019), 141, 9004-9017, PubMed 31117653 , DOI 10.1021/jacs.9b03116 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27836 released on 2019-03-13
Title Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain

Related entities 1. myosin-VI, : 1 : 2 : 2 : 1 : 31 entities Detail

Interaction partners 1. myosin-VI, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 4D 1H-15N-15N-1H HMQC-NOESY-TROSY-HSQC

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

2 TROSY-HSQC

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

3 3D TROSY-HNCACB

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

4 ARTSY-J

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

5 WEX-III TROSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 7.8, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

6 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

7 E.COSY-HSQC-TROSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

8 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13-; U-2H I13C MT-DOTA-Lu/Tm, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	entity_1	[U-15N; U-13C; U-2H]		1 mM

9 Heteronuclear TROSY-based relaxation

Instrument

Bruker 800-MHz Bruker - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

10 Heteronuclear TROSY-based relaxation

Instrument

Bruker 600-MHz Bruker - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

11 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30591_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30591_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	252		98.5 (chain: 1, length: 68)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 68, Sequence coverage: 98.5%
- Total number of assignments
  - ¹H chemical shifts: 67
  - ¹⁵N chemical shifts: 67
  - ¹³C chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	497	67	13.5
¹⁵N chemical shifts	89	67	75.3
¹³C chemical shifts	312	118	37.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	136	67	49.3
¹⁵N chemical shifts	68	67	98.5
¹³C chemical shifts	136	59	43.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	361	0	0.0
¹⁵N chemical shifts	21	0	0.0
¹³C chemical shifts	176	59	33.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	0	0.0
¹³C chemical shifts	14	3	21.4

Keywords MOTOR PROTEIN, alpha-helical domain, myosin-VI

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.594 seconds)

BMRB: 30591

Sample #1

Sample #2

Sample #3

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. myosin-VI, : 1 : 2 : 2 : 1 : 31 entities Detail

Interaction partners 1. myosin-VI, : 2 interactors Detail

Experiments performed 11 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail