BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	31.5 % (278 of 883)	17.1 % (85 of 497)	40.4 % (126 of 312)	90.5 % (67 of 74)
Backbone	64.5 % (263 of 408)	52.2 % (71 of 136)	61.3 % (125 of 204)	98.5 % (67 of 68)
Sidechain	13.8 % (75 of 543)	3.9 % (14 of 361)	34.7 % (61 of 176)	0.0 % (0 of 6)
Methyl	13.6 % (3 of 22)	0.0 % (0 of 11)	27.3 % (3 of 11)

1. entity 1

KQQEEEAERL RRIQEEMEKE RKRREEDEQR RRKEEEERRM KLEMEAKRKQ EEEERKKRED DEKRIQAE

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

Sample #3

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13-; U-2H I13C MT-DOTA-Lu/Tm, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	entity_1	[U-15N; U-13C; U-2H]		1 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6OBI, Strand ID: A Detail

Release date

2019-06-03

Citation

Remarkable Rigidity of the Single α-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy
Barnes, C.A., Shen, Y., Ying, J., Takagi, Y., Torchia, D.A., Sellers, J.R., Bax, A.
J. Am. Chem. Soc. (2019), 141, 9004-9017, PubMed 31117653 , DOI 10.1021/jacs.9b03116 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 27836 released on 2019-03-13
Title Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain

Related entities 1. myosin-VI, : 1 : 2 : 2 : 1 : 31 entities Detail

Interaction partners 1. myosin-VI, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 4D 1H-15N-15N-1H HMQC-NOESY-TROSY-HSQC

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

2 TROSY-HSQC

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

3 3D TROSY-HNCACB

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

4 ARTSY-J

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

5 WEX-III TROSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 7.8, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

6 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

7 E.COSY-HSQC-TROSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

8 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13-; U-2H I13C MT-DOTA-Lu/Tm, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	entity_1	[U-15N; U-13C; U-2H]		1 mM

9 Heteronuclear TROSY-based relaxation

Instrument

Bruker 800-MHz Bruker - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

10 Heteronuclear TROSY-based relaxation

Instrument

Bruker 600-MHz Bruker - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-2H 15N-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	entity_1	[U-15N; U-2H]		1 mM

11 ARTSY

Instrument

Bruker 900-MHz Bruker Avance-II - 900 MHz

Sample

State anisotropic, Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.3, Details 1 mM U-15N; U-13C; U-2H 15N-, 13C-, and 2H-isotopically enriched, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C; U-2H]		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30591_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30591_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	252		98.5 (chain: 1, length: 68)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 68, Sequence coverage: 98.5%
- Total number of assignments
  - ¹H chemical shifts: 67
  - ¹⁵N chemical shifts: 67
  - ¹³C chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	497	67	13.5
¹⁵N chemical shifts	89	67	75.3
¹³C chemical shifts	312	118	37.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	136	67	49.3
¹⁵N chemical shifts	68	67	98.5
¹³C chemical shifts	136	59	43.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	361	0	0.0
¹⁵N chemical shifts	21	0	0.0
¹³C chemical shifts	176	59	33.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	0	0.0
¹³C chemical shifts	14	3	21.4

Keywords MOTOR PROTEIN, alpha-helical domain, myosin-VI

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Found 1 Document (0.535 seconds)

BMRB: 30591

Sample #1

Sample #2

Sample #3

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. myosin-VI, : 1 : 2 : 2 : 1 : 31 entities Detail

Interaction partners 1. myosin-VI, : 2 interactors Detail

Experiments performed 11 experiments Detail

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Chemical shift validation 3 contents Detail