BMRBj - BMREntryMaster

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F2I5G8 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
1	Moronecidin	[15N]-F2I5G8	10 (±1.0) mM
2	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
3	Cholesterol	natural abundance	80 (±15.0) mM

Sample #2

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-V10G13I16 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
4	Moronecidin	[15N]-V10G13I16	10 (±1.0) mM
5	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
6	Cholesterol	natural abundance	80 (±15.0) mM

Sample #3

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-L19V20 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
7	Moronecidin	[15N]-L19V20	10 (±1.0) mM
8	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
9	Cholesterol	natural abundance	80 (±15.0) mM

Sample #4

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-I9T15 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
10	Moronecidin	[15N]-I9T15	10 (±1.0) mM
11	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
12	Cholesterol	natural abundance	80 (±15.0) mM

Sample #5

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6V12 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
13	Moronecidin	[15N]-F6V12	10 (±1.0) mM
14	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
15	Cholesterol	natural abundance	80 (±15.0) mM

Sample #6

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R18 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
16	Moronecidin	[15N]-R18	10 (±1.0) mM
17	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
18	Cholesterol	natural abundance	80 (±1.0) mM

Sample #7

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H4 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
19	Moronecidin	[15N]-H4	10 (±1.0) mM
20	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
21	Cholesterol	natural abundance	80 (±15.0) mM

Sample #8

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-K14 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
22	Moronecidin	[15N]-K14	10 (±1.0) mM
23	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
24	Cholesterol	natural abundance	80 (±15.0) mM

Sample #9

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R7 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
25	Moronecidin	[15N]-R7	10 (±1.0) mM
26	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
27	Cholesterol	natural abundance	80 (±15.0) mM

Sample #10

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H11 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
28	Moronecidin	[15N]-H11	10 (±1.0) mM
29	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
30	Cholesterol	natural abundance	80 (±15.0) mM

Sample #11

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H17 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
31	Moronecidin	[15N]-H17	10 (±1.0) mM
32	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
33	Cholesterol	natural abundance	80 (±15.0) mM

Sample #12

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-T21G22 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
34	Moronecidin	[15N]-T21G22	10 (±1.0) mM
35	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
36	Cholesterol	natural abundance	80 (±15.0) mM

Sample #13

Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H3 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
37	Moronecidin	[15N]-H3	10 (±1.0) mM
38	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
39	Cholesterol	natural abundance	80 (±15.0) mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6PF0, Strand ID: A Detail

Release date

2019-10-08

Citation

The host-defense peptide piscidin P1 reorganizes lipid domains in membranes and decreases activation energies in mechanosensitive ion channels
Comert, F., Heinrich, F., Chowdhury, A., Schoeneck, M., Darling, C., Anderson, K.W., Libardo, M., Angeles-Boza, A.M., Silin, V., Cotten, M.L., Mihailescu, M.
J. Biol. Chem. (2019), 11, 12620-12620, PubMed 31619519 , DOI 10.1074/jbc.RA119.010232 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30615 released on 2019-10-08
Title SOLID-STATE NMR STRUCTURE OF PISCIDIN 3 IN ALIGNED 4:1 PHOSPHATIDYLCHOLINE/CHOLESTEROL LIPID BILAYERS

Related entities 1. Moronecidin, : 1 : 7 : 7 entities Detail

Experiments performed 13 experiments Detail

1 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F2I5G8 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
1	Moronecidin	[15N]-F2I5G8	10 (±1.0) mM
2	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
3	Cholesterol	natural abundance	80 (±15.0) mM

2 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-L19V20 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
7	Moronecidin	[15N]-L19V20	10 (±1.0) mM
8	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
9	Cholesterol	natural abundance	80 (±15.0) mM

3 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-I9T15 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
10	Moronecidin	[15N]-I9T15	10 (±1.0) mM
11	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
12	Cholesterol	natural abundance	80 (±15.0) mM

4 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-F6V12 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
13	Moronecidin	[15N]-F6V12	10 (±1.0) mM
14	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
15	Cholesterol	natural abundance	80 (±15.0) mM

5 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R18 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
16	Moronecidin	[15N]-R18	10 (±1.0) mM
17	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
18	Cholesterol	natural abundance	80 (±1.0) mM

6 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-V10G13I16 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
4	Moronecidin	[15N]-V10G13I16	10 (±1.0) mM
5	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
6	Cholesterol	natural abundance	80 (±15.0) mM

7 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H4 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
19	Moronecidin	[15N]-H4	10 (±1.0) mM
20	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
21	Cholesterol	natural abundance	80 (±15.0) mM

8 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz Rennselaer Polytechnic Institute

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-K14 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
22	Moronecidin	[15N]-K14	10 (±1.0) mM
23	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
24	Cholesterol	natural abundance	80 (±15.0) mM

9 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 900 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-R7 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
25	Moronecidin	[15N]-R7	10 (±1.0) mM
26	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
27	Cholesterol	natural abundance	80 (±15.0) mM

10 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H11 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
28	Moronecidin	[15N]-H11	10 (±1.0) mM
29	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
30	Cholesterol	natural abundance	80 (±15.0) mM

11 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H17 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
31	Moronecidin	[15N]-H17	10 (±1.0) mM
32	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
33	Cholesterol	natural abundance	80 (±15.0) mM

12 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-T21G22 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
34	Moronecidin	[15N]-T21G22	10 (±1.0) mM
35	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
36	Cholesterol	natural abundance	80 (±15.0) mM

13 15N_1H solid-state de-HETCOR

Instrument

Bruker AVANCE - 600 MHz National High Magnetic Field Laboratory

Sample

State anisotropic, Solvent system H2O 100%; phosphate buffer 40 mM pH 7.4, Pressure 1 atm, Temperature 305 K, pH 7.4, Details 10 mM [15N]-H3 Moronecidin, 320 mM 1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine, 80 mM Cholesterol, H2O 100%; phosphate buffer 40 mM pH 7.4

#	Name	Isotope labeling	Concentration
37	Moronecidin	[15N]-H3	10 (±1.0) mM
38	1-palmitoyl-2-oleoyl-sn-glycero-phosphatidylcholine	natural abundance	320 (±50.0) mM
39	Cholesterol	natural abundance	80 (±15.0) mM

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	21		91.3 (chain: 1, length: 23)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	25	21	84.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	23	21	91.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	2	0	0.0

Keywords AMPHIPATHIC, ANTI HIV-1, ANTICANCER PEPTIDE, ANTIMICROBIAL PEPTIDE, ANTIMICROBIAL PROTEIN, BACTERIAL CELL MEMBRANE MIMIC, CATIONIC, HELICAL, HISTIDINE RICH, LIPID BILAYERS

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30616_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (0.411 seconds)

BMRB: 30616

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Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Moronecidin, : 1 : 7 : 7 entities Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail