Solution NMR Structure of the Coiled-coil BRCA1-PALB2 Heterodimer
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.2 % (1172 of 1538) | 81.7 % (664 of 813) | 67.9 % (396 of 583) | 78.9 % (112 of 142) |
Backbone | 75.1 % (569 of 758) | 87.1 % (222 of 255) | 62.6 % (238 of 380) | 88.6 % (109 of 123) |
Sidechain | 77.8 % (703 of 904) | 79.2 % (442 of 558) | 78.9 % (258 of 327) | 15.8 % (3 of 19) |
Aromatic | 15.6 % (14 of 90) | 15.6 % (7 of 45) | 15.6 % (7 of 45) | |
Methyl | 92.7 % (102 of 110) | 92.7 % (51 of 55) | 92.7 % (51 of 55) |
1. entity 1
MNLSEDCSQS DILTTQQRAT MKYNLIKLQQ EMAHLEAVLE QRGNQPSGHS PSLEHHHHHH2. entity 2
MEELSGKPLS YAEKEKLKEK LAFLKKEYSR TLARLQRAKR AEKAKNSKKA IEDGVPQPEA LEHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.5 mM [U-100% 13C; U-100% 15N] PALB2cc, 3 mM BRCA1cc, 20 mM MES, 200 mM sodium chloride, 10 mM DTT, 5 mM CaCl2, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PALB2cc | [U-100% 13C; U-100% 15N] | 1.5 mM | |
2 | BRCA1cc | natural abundance | 3 mM | |
3 | MES | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 200 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | CaCl2 | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.5 mM [U-100% 13C; U-100% 15N] BRCA1cc, 3 mM PALB2cc, 20 mM MES, 200 mM sodium chloride, 10 mM DTT, 5 mM CaCl2, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | PALB2cc | natural abundance | 3 mM | |
8 | BRCA1cc | [U-100% 13C; U-100% 15N] | 1.5 mM | |
9 | MES | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 200 mM | |
11 | DTT | natural abundance | 10 mM | |
12 | CaCl2 | natural abundance | 5 mM |