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Found 1 Document (0.262 seconds)

BMRB: 30884

Solution NMR Structure of PawL-Derived Peptide PLP-29

Authors

Payne, C.D., Rosengren, K.J.

Assembly

PLP-29

Entity

1. PLP-29 (polymer, Thiol state: not present), 8 monomers, 852.9300 Da Detail

GYFPVGVD

Formula weight

852.93 Da

Source organism

Steirodiscus tagetes

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 68.2 %, Completeness (bb): 76.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.2 % (60 of 88)	79.5 % (35 of 44)	51.4 % (19 of 37)	85.7 % (6 of 7)
Backbone	76.1 % (35 of 46)	88.2 % (15 of 17)	63.6 % (14 of 22)	85.7 % (6 of 7)
Sidechain	64.6 % (31 of 48)	74.1 % (20 of 27)	52.4 % (11 of 21)
Aromatic	11.1 % (2 of 18)	22.2 % (2 of 9)	0.0 % (0 of 9)
Methyl	100.0 % (8 of 8)	100.0 % (4 of 4)	100.0 % (4 of 4)

1. entity 1

GYFPVGVD

Show sample condition

Sample

Solvent system 99.5% CD3CN, Pressure 1 atm, Temperature 298 K, pH 3.5, Details 4 mg/mL PLP-29, 99.5% CD3CN

#	Name	Isotope labeling	Type	Concentration
1	PLP-29	natural abundance		4 mg/mL

Release date

2021-03-16

Citation

Structural Characterization of the PawL-Derived Peptide Family, an Ancient Subfamily of Orbitides
Payne, C.D., Fisher, M.F., Mylne, J.S., Rosengren, K.J.
J. Nat. Prod. (2021), 84, 2914-2922, PubMed 34672199 , DOI 10.1021/acs.jnatprod.1c00672 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 30881 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-13
  BMRB: 30882 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-16
  BMRB: 30883 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-22
  BMRB: 30885 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-38
  BMRB: 30886 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-42
  BMRB: 30887 released on 2021-03-16
    Title Solution NMR Structure of PawL-Derived Peptide PLP-46

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	68		100.0 (chain: 1, length: 8)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	35	79.5
¹³C chemical shifts	37	19	51.4
¹⁵N chemical shifts	7	6	85.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	15	88.2
¹³C chemical shifts	16	8	50.0
¹⁵N chemical shifts	7	6	85.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	20	74.1
¹³C chemical shifts	21	11	52.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	4	100.0
¹³C chemical shifts	4	4	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	2	22.2
¹³C chemical shifts	9	0	0.0

Keywords AEP-processed, Cyclic, Orbitide, PLANT PROTEIN, PLP

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30884_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

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Found 1 Document (0.262 seconds)

BMRB: 30884

Sample

Entries sharing articles BMRB: 6 entries Detail

Experiments performed 8 experiments Detail

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Chemical shift validation 3 contents Detail