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cTnC-TnI chimera complexed with calcium
Authors
Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.
Assembly
Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle
Entity
1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1 (polymer), 129 monomers, 14204.98 Da Detail

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED


2. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity CA (non-polymer), 40.078 Da
Total weight
14245.059 Da
Max. entity weight
14204.98 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.5 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.5 % (1420 of 1457)97.4 % (737 of 757)97.9 % (548 of 560)96.4 % (135 of 140)
Backbone98.6 % (759 of 770)98.1 % (262 of 267)98.9 % (372 of 376)98.4 % (125 of 127)
Sidechain96.6 % (778 of 805)96.9 % (475 of 490)97.0 % (293 of 302)76.9 % (10 of 13)
Aromatic72.6 % (45 of 62)71.0 % (22 of 31)74.2 % (23 of 31)
Methyl100.0 % (134 of 134)100.0 % (67 of 67)100.0 % (67 of 67)

1. entity 1

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1TnC-TnI chimera[U-13C; U-15N]1 mM
2NaClnatural abundance50 mM
3Trisnatural abundance20 mM

Release date
2021-12-01
Citation
Structural and Thermodynamic Model for the Activation of Cardiac Troponin
Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.
Biochemistry (2022), 61, 741-748, PubMed 35349258 , DOI 10.1021/acs.biochem.2c00084 ,
Entries sharing articles BMRB: 5 entries Detail
  BMRB: 30966 released on 2022-01-02
    Title cTnC-TnI chimera complexed with A2
  BMRB: 30963 released on 2021-12-16
    Title NMR structure of cTnC-TnI chimera bound to calcium and A2
  BMRB: 30965 released on 2021-12-16
    Title cTnC-TnI chimera complexed with A1
  BMRB: 30962 released on 2021-12-08
    Title NMR structure of cTnC-TnI chimera bound to calcium and A1
  BMRB: 30968 released on 2021-12-01
    Title NMR solution structure TnC-TnI chimera
Related entities 1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1, : 1 : 5 : 11 entities Detail
Experiments performed 10 experiments Detail
nullKeywords METAL BINDING PROTEIN, calcium sensitizer, cardiac troponin