BMRB: 30962

NMR structure of cTnC-TnI chimera bound to calcium and A1

Authors

Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.

Assembly

Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle

Entity

1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1 (polymer), 129 monomers, 14204.98 Da Detail

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED

2. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity BQ6 (non-polymer), 446.495 Da
3. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity CA (non-polymer), 40.078 Da

Total weight

14691.554 Da

Max. entity weight

14204.98 Da

Entity Connection

na 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:ASP65:OD1	3:CA1:CA
2	na	sing	1:ASP65:OD2	3:CA1:CA
3	na	sing	1:ASP67:OD1	3:CA1:CA
4	na	sing	1:SER69:OG	3:CA1:CA
5	na	sing	1:THR71:O	3:CA1:CA
6	na	sing	1:GLU76:OE1	3:CA1:CA

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.0 % (1428 of 1457)	97.8 % (740 of 757)	97.9 % (548 of 560)	100.0 % (140 of 140)
Backbone	98.1 % (755 of 770)	97.8 % (261 of 267)	97.6 % (367 of 376)	100.0 % (127 of 127)
Sidechain	97.6 % (786 of 805)	97.3 % (477 of 490)	98.0 % (296 of 302)	100.0 % (13 of 13)
Aromatic	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)
Methyl	97.0 % (130 of 134)	97.0 % (65 of 67)	97.0 % (65 of 67)

1. entity 1

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] cTnC-cTnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cTnC-cTnI chimera	[U-13C; U-15N]		1 mM

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Release date

2021-12-08

Citation

Structural and Thermodynamic Model for the Activation of Cardiac Troponin
Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.
Biochemistry (2022), 61, 741-748, PubMed 35349258 , DOI 10.1021/acs.biochem.2c00084 , Abstract

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1, : 1 : 5 : 11 entities Detail

Experiments performed 11 experiments Detail