BMRBj - BMREntryMaster

Found 1 Document (0.597 seconds)

BMRB: 30967

7SXC

cTnC-TnI chimera complexed with calcium

Authors

Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.

Assembly

Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle

Entity

1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1 (polymer), 129 monomers, 14204.98 Da Detail

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED

2. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity CA (non-polymer), 40.078 Da

Total weight

14245.059 Da

Max. entity weight

14204.98 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.5 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.5 % (1420 of 1457)	97.4 % (737 of 757)	97.9 % (548 of 560)	96.4 % (135 of 140)
Backbone	98.6 % (759 of 770)	98.1 % (262 of 267)	98.9 % (372 of 376)	98.4 % (125 of 127)
Sidechain	96.6 % (778 of 805)	96.9 % (475 of 490)	97.0 % (293 of 302)	76.9 % (10 of 13)
Aromatic	72.6 % (45 of 62)	71.0 % (22 of 31)	74.2 % (23 of 31)
Methyl	100.0 % (134 of 134)	100.0 % (67 of 67)	100.0 % (67 of 67)

1. entity 1

GDDIYKAAVE QLTEEQKNEF KAAFDIFVLG AEDGCISTKE LGKVMRMLGQ NPTPEELQEM IDEVDEDGSG TVDFDEFLVM MVRCMKDDSK GAGAGVRISA DAMMQALLGA RAKESLDLRA HLKQVKKED

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

Release date

2021-12-01

Citation

Structural and Thermodynamic Model for the Activation of Cardiac Troponin
Poppe, L., Hartman, J.J., Romero, A., Reagan, J.D.
Biochemistry (2022), 61, 741-748, PubMed 35349258 , DOI 10.1021/acs.biochem.2c00084 , Abstract

Entries sharing articles BMRB: 5 entries Detail

  BMRB: 30966 released on 2022-01-02
    Title cTnC-TnI chimera complexed with A2
  BMRB: 30963 released on 2021-12-16
    Title NMR structure of cTnC-TnI chimera bound to calcium and A2
  BMRB: 30965 released on 2021-12-16
    Title cTnC-TnI chimera complexed with A1
  BMRB: 30962 released on 2021-12-08
    Title NMR structure of cTnC-TnI chimera bound to calcium and A1
  BMRB: 30968 released on 2021-12-01
    Title NMR solution structure TnC-TnI chimera

Related entities 1. Troponin C, slow skeletal and cardiac muscles,Troponin I, cardiac muscle, entity 1, : 1 : 5 : 11 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

3 3D 1H-15N NOESY

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

4 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

5 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

6 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

7 CBCANH

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

8 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

9 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

10 3D HNCO

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1 mM [U-13C; U-15N] TnC-TnI chimera, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	TnC-TnI chimera	[U-13C; U-15N]		1 mM
2	NaCl	natural abundance		50 mM
3	Tris	natural abundance		20 mM

nullKeywords METAL BINDING PROTEIN, calcium sensitizer, cardiac troponin