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Found 1 Document (0.747 seconds)

BMRB: 30971

7T1N

Solution structure of 7SK stem-loop 1 with HEXIM Arginine Rich Motif

Authors

Pham, V.V., Gao, M., D'Souza, V.M.

Assembly

HEXIM Arginine Rich Motif/RNA Complex

Entity

1. HEXIM Arginine Rich Motif/RNA Complex, entity 1 (polymer, Thiol state: not present), 56 monomers, 18049.61 Da Detail

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. HEXIM Arginine Rich Motif/RNA Complex, entity 2 (polymer, Thiol state: not present), 19 monomers, 2276.609 Da Detail

GISYGRQLGK KKHRRRAHQ

Total weight

20326.219 Da

Max. entity weight

18049.61 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 33.7 %, Completeness (bb): 19.6 % Detail

Polymer type: polyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.7 % (386 of 1144)	36.5 % (229 of 627)	26.7 % (123 of 460)	59.6 % (34 of 57)
Backbone	19.6 % (143 of 730)	21.2 % (80 of 377)	15.9 % (53 of 334)	52.6 % (10 of 19)
Sidechain	58.1 % (250 of 430)	59.6 % (149 of 250)	54.2 % (77 of 142)	63.2 % (24 of 38)
Aromatic	75.6 % (201 of 266)	91.0 % (121 of 133)	55.4 % (56 of 101)	75.0 % (24 of 32)
Methyl	0.0 % (0 of 10)	0.0 % (0 of 5)	0.0 % (0 of 5)

1. entity 1

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. entity 2

GISYGRQLGK KKHRRRAHQ

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
1	HEXIM	natural abundance	0.5 (±0.2) mM
2	7SK	natural abundance	0.5 (±0.2) mM
3	NaCl	natural abundance	70 mM
4	EDTA	natural abundance	0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	HEXIM	natural abundance	0.5 (±0.2) mM
6	7SK	natural abundance	0.5 (±0.2) mM
7	NaCl	natural abundance	70 mM
8	EDTA	natural abundance	0.1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM [U-100% 13C; U-100% 15N] HEXIM, 0.5 mM 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	HEXIM	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
10	7SK	natural abundance	0.5 (±0.2) mM
11	NaCl	natural abundance	70 mM
12	EDTA	natural abundance	0.1 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM [U-100% 13C; U-100% 15N] 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
13	HEXIM	natural abundance	0.5 (±0.2) mM
14	7SK	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
15	NaCl	natural abundance	70 mM
16	EDTA	natural abundance	0.1 mM

Sample #5

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM [U-100% 13C; U-100% 15N] 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
17	HEXIM	natural abundance	0.5 (±0.2) mM
18	7SK	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
19	NaCl	natural abundance	70 mM
20	EDTA	natural abundance	0.1 mM

Sample #6

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM [U-100% 13C; U-100% 15N] HEXIM, 0.5 mM 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
21	HEXIM	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
22	7SK	natural abundance	0.5 (±0.2) mM
23	NaCl	natural abundance	70 mM
24	EDTA	natural abundance	0.1 mM

Release date

2021-12-29

Citation

A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA
Pham, V.V., Gao, M., Meagher, J.L., Smith, J.L., D'Souza, V.M.
Commun. Biol. (2022), 5, 819-819, PubMed 35970937 , DOI 10.1038/s42003-022-03734-w , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30973 released on 2022-02-06
    Title Solution structure of 7SK stem-loop 1 with HIV-1 Tat Finland Arginine Rich Motif
  BMRB: 30972 released on 2022-02-01
    Title Solution structure of 7SK stem-loop 1 with HIV-1 Tat Subtype G Arginine Rich Motif

Related entities 1. HEXIM Arginine Rich Motif/RNA Complex, entity 1, : 1 : 2 : 6 entities Detail

Related entities 2. HEXIM Arginine Rich Motif/RNA Complex, entity 2, : 1 : 1 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
1	HEXIM	natural abundance	0.5 (±0.2) mM
2	7SK	natural abundance	0.5 (±0.2) mM
3	NaCl	natural abundance	70 mM
4	EDTA	natural abundance	0.1 mM

2 2D 1H-1H NOESY

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	HEXIM	natural abundance	0.5 (±0.2) mM
6	7SK	natural abundance	0.5 (±0.2) mM
7	NaCl	natural abundance	70 mM
8	EDTA	natural abundance	0.1 mM

3 2D 1H-13C HSQC

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM [U-100% 13C; U-100% 15N] HEXIM, 0.5 mM 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	HEXIM	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
10	7SK	natural abundance	0.5 (±0.2) mM
11	NaCl	natural abundance	70 mM
12	EDTA	natural abundance	0.1 mM

4 2D 1H-13C HSQC

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM [U-100% 13C; U-100% 15N] 7SK, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
13	HEXIM	natural abundance	0.5 (±0.2) mM
14	7SK	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
15	NaCl	natural abundance	70 mM
16	EDTA	natural abundance	0.1 mM

5 2D 1H-13C HSQC aliphatic

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM HEXIM, 0.5 mM [U-100% 13C; U-100% 15N] 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
17	HEXIM	natural abundance	0.5 (±0.2) mM
18	7SK	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
19	NaCl	natural abundance	70 mM
20	EDTA	natural abundance	0.1 mM

6 2D 1H-13C HSQC aliphatic

Instrument

Bruker DMX - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM [U-100% 13C; U-100% 15N] HEXIM, 0.5 mM 7SK, 100% D2O

#	Name	Isotope labeling	Concentration
21	HEXIM	[U-100% 13C; U-100% 15N]	0.5 (±0.2) mM
22	7SK	natural abundance	0.5 (±0.2) mM
23	NaCl	natural abundance	70 mM
24	EDTA	natural abundance	0.1 mM

nullKeywords 7SK, HIV-1, TRANSLATION, Tat

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Found 1 Document (0.747 seconds)

BMRB: 30971

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. HEXIM Arginine Rich Motif/RNA Complex, entity 1, : 1 : 2 : 6 entities Detail

Related entities 2. HEXIM Arginine Rich Motif/RNA Complex, entity 2, : 1 : 1 entities Detail

Experiments performed 6 experiments Detail

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