BMRB: 30973

Solution structure of 7SK stem-loop 1 with HIV-1 Tat Finland Arginine Rich Motif

Authors

Pham, V.V., Gao, M., D'Souza, V.M.

Assembly

Tat Finland Arginine Rich Motif/RNA Complex

Entity

1. Tat Finland Arginine Rich Motif/RNA Complex, entity 1 (polymer, Thiol state: not present), 56 monomers, 18049.61 Da Detail

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. Tat Finland Arginine Rich Motif/RNA Complex, entity 2 (polymer, Thiol state: not present), 17 monomers, 2134.456 Da Detail

GISYGRKKRK HRRRAHQ

Total weight

20184.066 Da

Max. entity weight

18049.61 Da

Source organism

Homo sapiens , HIV-1 06TG.HT008

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 35.4 %, Completeness (bb): 18.5 % Detail

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Polymer type: polyribonucleotide polypeptide(L)

	Total	1H
All	35.4 % (219 of 618)	35.4 % (219 of 618)
Backbone	18.5 % (69 of 372)	18.5 % (69 of 372)
Sidechain	61.0 % (150 of 246)	61.0 % (150 of 246)
Aromatic	89.5 % (119 of 133)	89.5 % (119 of 133)
Methyl	0.0 % (0 of 3)	0.0 % (0 of 3)

1. entity 1

GGGAUCUGUC ACCCCAUUGA UCGCCAGUGG CUGAUCUGGC UGGCUAGGCG GGUCCC

2. entity 2

GISYGRKKRK HRRRAHQ

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM 7SK-SL1, 0.5 mM Tat Fin, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	7SK-SL1	natural abundance		0.5 (±0.2) mM
2	Tat Fin	natural abundance		0.5 (±0.2) mM
3	Phosphate	natural abundance		10 mM
4	NaCl	natural abundance		70 mM
5	EDTA	natural abundance		0.1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 100000 Pa, Temperature 298 K, pH 5.6, Details 0.5 mM 7SK-SL1, 0.5 mM Tat Fin, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
6	7SK-SL1	natural abundance		0.5 (±0.2) mM
7	Tat Fin	natural abundance		0.5 (±0.2) mM
8	Phosphate	natural abundance		10 mM
9	NaCl	natural abundance		70 mM
10	EDTA	natural abundance		0.1 mM

Hide sample codition

Release date

2022-02-06

Citation

A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA
Pham, V.V., Gao, M., Meagher, J.L., Smith, J.L., D'Souza, V.M.
Commun. Biol. (2022), 5, 819-819, PubMed 35970937 , DOI 10.1038/s42003-022-03734-w , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Tat Finland Arginine Rich Motif/RNA Complex, entity 1, : 1 : 2 : 6 entities Detail

Related entities 2. Tat Finland Arginine Rich Motif/RNA Complex, entity 2, : 1 : 1 : 6 entities Detail

Experiments performed 2 experiments Detail